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[Effect of alpha-difluoromethylornithine on growth characteristics and expression of ALT-04ag gene of human lung carcinoma cells].

AbstractOBJECTIVE:
To study the effect of polyamine biosynthesis inhibition on growth characteristics of human lung carcinoma cells and its correlation with the expression of human lung carcinoma associated antigen ALT-04ag gene.
METHODS:
The gene expression was detected by RT-PCR and immunocytochemical tests. The cell growth characteristics were studied by cell growth curves, morphological observation, FCM analysis and DNA electrophoresis.
RESULTS:
Human lung squamous carcinoma cells L78 treated with 5 mmol/L alpha-difluoromethylornithine (DFMO) for 5 days showed significant growth inhibition and apoptosis induction. The mRNA and protein expressions of ALT-04ag gene in the cells were downregulated, while these changes resulted from DFMO treatment were prevented by provision of DFMO along with exogenous putrescine.
CONCLUSION:
The effect of polyamine biosynthesis inhibition induced by DFMO restrains the growth characteristics and promotes apoptosis of human lung carcinoma L78 cells, which is associated with down regulation of ALT-04ag gene expression.
AuthorsZhi-hua Chen, Hai-ping Ke, Hong-yan Li, Qing Fang, Shu-lan Cao, Mu-zhen Fan
JournalZhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 24 Issue 4 Pg. 377-80 (Aug 2002) ISSN: 1000-503X [Print] China
PMID12905657 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Antigens, Neoplasm
  • Oncogene Proteins
  • RNA, Messenger
  • Eflornithine
Topics
  • Antigens, Neoplasm (biosynthesis, genetics)
  • Apoptosis
  • Carcinoma, Squamous Cell (pathology)
  • Cell Division
  • Down-Regulation
  • Eflornithine (pharmacology)
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms (pathology)
  • Oncogene Proteins (genetics)
  • RNA, Messenger (biosynthesis, genetics)
  • Tumor Cells, Cultured

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