Abstract | OBJECTIVE: METHODS: The gene expression was detected by RT-PCR and immunocytochemical tests. The cell growth characteristics were studied by cell growth curves, morphological observation, FCM analysis and DNA electrophoresis. RESULTS: Human lung squamous carcinoma cells L78 treated with 5 mmol/L alpha-difluoromethylornithine (DFMO) for 5 days showed significant growth inhibition and apoptosis induction. The mRNA and protein expressions of ALT-04ag gene in the cells were downregulated, while these changes resulted from DFMO treatment were prevented by provision of DFMO along with exogenous putrescine. CONCLUSION: The effect of polyamine biosynthesis inhibition induced by DFMO restrains the growth characteristics and promotes apoptosis of human lung carcinoma L78 cells, which is associated with down regulation of ALT-04ag gene expression.
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Authors | Zhi-hua Chen, Hai-ping Ke, Hong-yan Li, Qing Fang, Shu-lan Cao, Mu-zhen Fan |
Journal | Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
(Zhongguo Yi Xue Ke Xue Yuan Xue Bao)
Vol. 24
Issue 4
Pg. 377-80
(Aug 2002)
ISSN: 1000-503X [Print] China |
PMID | 12905657
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Antigens, Neoplasm
- Oncogene Proteins
- RNA, Messenger
- Eflornithine
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Topics |
- Antigens, Neoplasm
(biosynthesis, genetics)
- Apoptosis
- Carcinoma, Squamous Cell
(pathology)
- Cell Division
- Down-Regulation
- Eflornithine
(pharmacology)
- Gene Expression Regulation, Neoplastic
- Humans
- Lung Neoplasms
(pathology)
- Oncogene Proteins
(genetics)
- RNA, Messenger
(biosynthesis, genetics)
- Tumor Cells, Cultured
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