The effects of caffeic acid phenethyl ester on tissue damage in lung after hindlimb ischemia-reperfusion.

Abstract

AIM:

The aim of this study was to investigate the effects of caffeic acid phenethyl ester (CAPE) on the lungs as a remote organ after performing hindlimb ischemia-reperfusion (I/R) and by assessing biochemical and histopathological analysis.

METHODS:

The animals were divided into three groups: control, I/R, and I/R with CAPE. I/R period for 8 h was performed on the right hindlimb of all the anesthesied rats in I/R and CAPE with I/R group. In the CAPE with I/R group, the animals received CAPE 10 microM by intraperitoneal injection 1h before the reperfusion. The animals in the control and I/R groups received a similar volume of saline solution by means of intraperitoneal injection. At the end of the reperfusion period, a midsternotomy was performed. Blood, bronchoalveolar lavage (BAL) and lung tissue were obtained, and were used for biochemical and histopathological examination.

RESULTS:

The tissue and serum malondyaldehyde levels were significantly lower in the control (P=0.0001 and 0.001, respectively) and in the CAPE with I/R groups (P=0.0001 and 0.003, respectively) compared to the I/R group. Tissue Na(+)-K(+) ATPase activity in the CAPE with I/R group was significantly higher than in the I/R group (P=0.0001). Reduced activity was found in the I/R group compared to the control group (P=0.0001). Myeloperoxidase activity (P=0.001) and protein concentration (P=0.034) in BAL were significantly reduced in CAPE-treated animals when compared with the I/R group. A decreased activity and protein concentration were found in the control group compared to the I/R group (P=0.0001 and 0.024, respectively). The lungs of the I/R group displayed intense peribronchial and perivascular leukocytic infiltration in histopathological examination compared to the CAPE with I/R group (P<0.05).

CONCLUSION:

CAPE seems to be effective in protecting remote organ injury caused by increased oxidative stress and neutrophil accumulation that results from an I/R injury.

• Authors: Mukadder Calikoglu, Lulufer Tamer, Nehir Sucu, Banu Coskun, Bahadir Ercan, Ali Gul, Ilker Calikoglu, Arzu Kanik
• Journal: Pharmacological research (Pharmacol Res) Vol. 48 Issue 4 Pg. 397-403 (Oct 2003) ISSN: 1043-6618 [Print] Netherlands
• PMID: 12902211 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Caffeic Acids
• Proteins
• Malondialdehyde
• Peroxidase
• Sodium-Potassium-Exchanging ATPase
• caffeic acid phenethyl ester
• Phenylethyl Alcohol

Topics

• Animals
• Bronchoalveolar Lavage (methods)
• Bronchoalveolar Lavage Fluid (chemistry, cytology)
• Caffeic Acids (administration & dosage, pharmacokinetics, therapeutic use)
• Capillary Permeability (drug effects)
• Hindlimb (blood supply, injuries, physiopathology)
• Injections, Intraperitoneal
• Lung (chemistry, drug effects, ultrastructure)
• Male
• Malondialdehyde (antagonists & inhibitors, blood)
• Neutrophil Infiltration (drug effects)
• Peroxidase (antagonists & inhibitors, chemistry, drug effects)
• Phenylethyl Alcohol (administration & dosage, analogs & derivatives, pharmacokinetics, therapeutic use)
• Proteins (antagonists & inhibitors, chemistry)
• Rats
• Rats, Wistar
• Reperfusion Injury (complications, drug therapy, physiopathology)
• Respiratory Distress Syndrome (complications, drug therapy, physiopathology)
• Sodium-Potassium-Exchanging ATPase (antagonists & inhibitors, chemistry, drug effects)
• Time Factors
• Tourniquets (adverse effects)
• Turkey