Expression levels of hsc70 and hsp60 are developmentally regulated during B-cell maturation and not associated to childhood c-ALL at presentation or relapse.

Abstract

Heat shock proteins are potent regulators of apoptosis, and so they may also be involved in normal cellular differentiation and cancerogenesis. We used quantitative two-dimensional gel electrophoresis for determining whether either the constitutive chaperonic heat shock cognate protein 70 (hsc70) or heat shock protein 60 (hsp60) contribute to B-cell differentiation and leukemogenesis. We compared the expression of these hsps in normal peripheral blood (PB) CD19+ B-cells, in pediatric bone marrow (BM) CD19+ CD10+ B-cell precursors (BCPs) from normal donors, and in BCPs from common acute lymphoblastic leukemia (c-ALL) patients at diagnosis and at relapse. We found that the mean levels of hsc70 in c-ALL BCPs at initial presentation and at relapse failed to differ significantly. Likewise, they failed to differ significantly from the level in high-expressing normal BM BCPs or from that in low-expressing PB B-cells. Mean levels of hsp60 expression in c-ALL BCPs at initial presentation and at relapse were similar and not distinguishable from that in normal BM BCPs, however, elevated (by a factor of 2-3) compared with that in PB B-cells. Hsc70 and Hsp60 expressions were increased (by a factor of 2 of mean levels) in populations of normal BM BCPs as compared with populations of PB B-cells. Thus, no abnormal levels of hsc70 and hsp60 were detectable in populations of pediatric c-ALL BCPs neither at diagnosis nor at relapse. In contrast, our data were in support of developmentally regulated levels of hsc70 and hsp60 expression during B-cell ontogenesis.

• Authors: Peder Skov Wehner, Bendt Nielsen, Marianne Hokland
• Journal: European journal of haematology (Eur J Haematol) Vol. 71 Issue 2 Pg. 100-8 (Aug 2003) ISSN: 0902-4441 [Print] England
• PMID: 12890148 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Chaperonin 60
• HSC70 Heat-Shock Proteins
• HSP70 Heat-Shock Proteins
• HSPA8 protein, human

Topics

• B-Lymphocytes (chemistry, pathology)
• Biomarkers (analysis)
• Case-Control Studies
• Cell Differentiation
• Chaperonin 60 (analysis)
• Electrophoresis, Gel, Two-Dimensional
• Gene Expression Regulation
• Gene Expression Regulation, Developmental
• Gene Expression Regulation, Leukemic
• HSC70 Heat-Shock Proteins
• HSP70 Heat-Shock Proteins (analysis)
• Humans
• Phenotype
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (etiology, metabolism, pathology)
• Recurrence