Abstract |
Tie-2, a tyrosine kinase receptor, is essential for vascular integrity by regulating cellular adhesion between pericytes and endothelial cells. The aim of this study was to identify sites of expression of Tie-2 other than the vasculature. Tie-2 expression was first detected in human colon by Western blotting and reverse-transcription-polymerase chain reaction (RT-PCR) in tissue extracts. The presence of the Tie-2 mRNA and protein was detected by immunohistochemistry and in situ hybridization in cells of the colon myenteric and submucosal plexus, in both neuronal and Schwann cells. Tie-2 protein was also found in the nervous system of the female urogenital tract. In the human sciatic nerve and schwannoma, RT-PCR, Western blotting and immunohistochemistry analysis further confirmed the presence of Tie-2 mRNA and protein in non-autonomic peripheral nervous tissue. In conclusion, using several approaches and tissues we have demonstrated the presence of Tie-2 in human peripheral and autonomic nervous tissue, suggesting a role for Tie-2 in neural tissue. Thus, attempts to disrupt the tumour vessels by manipulation of the Tie-2 system in tumours may result in side-effects in peripheral nerves.
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Authors | S Poncet, J-M Gasc, R C Janzer, S Meyer, L Juillerat-Jeanneret |
Journal | Neuropathology and applied neurobiology
(Neuropathol Appl Neurobiol)
Vol. 29
Issue 4
Pg. 361-9
(Aug 2003)
ISSN: 0305-1846 [Print] England |
PMID | 12887596
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Receptor Protein-Tyrosine Kinases
- Receptor, TIE-2
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Topics |
- Colon
(chemistry, cytology, innervation)
- Humans
- Immunohistochemistry
- In Situ Hybridization
- Myenteric Plexus
(chemistry, cytology, physiology)
- Neurons
(chemistry, physiology)
- RNA, Messenger
(analysis)
- Receptor Protein-Tyrosine Kinases
(analysis, genetics)
- Receptor, TIE-2
- Schwann Cells
(cytology, physiology)
- Submucous Plexus
(chemistry, cytology, physiology)
- Sural Nerve
(chemistry, cytology, physiology)
- Urogenital System
(chemistry, cytology, innervation)
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