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Expression of Tie-2 in human peripheral and autonomic nervous system.

Abstract
Tie-2, a tyrosine kinase receptor, is essential for vascular integrity by regulating cellular adhesion between pericytes and endothelial cells. The aim of this study was to identify sites of expression of Tie-2 other than the vasculature. Tie-2 expression was first detected in human colon by Western blotting and reverse-transcription-polymerase chain reaction (RT-PCR) in tissue extracts. The presence of the Tie-2 mRNA and protein was detected by immunohistochemistry and in situ hybridization in cells of the colon myenteric and submucosal plexus, in both neuronal and Schwann cells. Tie-2 protein was also found in the nervous system of the female urogenital tract. In the human sciatic nerve and schwannoma, RT-PCR, Western blotting and immunohistochemistry analysis further confirmed the presence of Tie-2 mRNA and protein in non-autonomic peripheral nervous tissue. In conclusion, using several approaches and tissues we have demonstrated the presence of Tie-2 in human peripheral and autonomic nervous tissue, suggesting a role for Tie-2 in neural tissue. Thus, attempts to disrupt the tumour vessels by manipulation of the Tie-2 system in tumours may result in side-effects in peripheral nerves.
AuthorsS Poncet, J-M Gasc, R C Janzer, S Meyer, L Juillerat-Jeanneret
JournalNeuropathology and applied neurobiology (Neuropathol Appl Neurobiol) Vol. 29 Issue 4 Pg. 361-9 (Aug 2003) ISSN: 0305-1846 [Print] England
PMID12887596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Messenger
  • Receptor Protein-Tyrosine Kinases
  • Receptor, TIE-2
Topics
  • Colon (chemistry, cytology, innervation)
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Myenteric Plexus (chemistry, cytology, physiology)
  • Neurons (chemistry, physiology)
  • RNA, Messenger (analysis)
  • Receptor Protein-Tyrosine Kinases (analysis, genetics)
  • Receptor, TIE-2
  • Schwann Cells (cytology, physiology)
  • Submucous Plexus (chemistry, cytology, physiology)
  • Sural Nerve (chemistry, cytology, physiology)
  • Urogenital System (chemistry, cytology, innervation)

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