Abstract |
The aim of the present study is to investigate whether Met416Val (M416V) polymorphism of glycogen synthase (GYS1) gene is associated with insulin resistance in type 2 diabetes. In 100 type 2 diabetic subjects (66 men and 34 women), the M416V polymorphism of GYS1 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as previously reported, and insulin resistance was assessed by euglycemic hyperinsulinemic clamp represented as M/I value, the mean of glucose infusion rate (M value) adjusted by steady state plasma insulin level. The means of age and body mass index (BMI) of the subjects were 53.1+/-11.6 (SD) years and 23.3+/-3.5 kg/m2. The allele frequencies of M416V polymorphism were 82.0% for MM, 16.0% for MV, and 2.0% for VV, and subjects were subsequently divided into V(+) group (n=18) and V(-) group (n=82) according to the presence or absence of V allele. There were no significant differences in age, BMI, blood pressure, fasting plasma glucose or insulin levels or glycosylated hemoglobin (HbA1c) levels between the V(+) and V(-) groups. No significant differences in either M or M/I value were found between the V(+) and V(-) groups (M value, 5.06+/-2.20 v 5.12+/-2.04 mg x kg(-1) x min(-1), P=.841; M/I value, 5.24+/-3.07 v 5.39+/-2.87 mg x kg(-1) x min(-1) x mU(-1) x L, P=.576). BMI showed the strongest independent contribution to M/I value, but the presence of V allele did not in multiple regression analysis. In conclusion, the M416V polymorphism of GYS1 gene is not associated with insulin resistance in type 2 diabetes.
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Authors | Koka Motoyama, Masanori Emoto, Hideki Tahara, Miyoko Komatsu, Tetsuo Shoji, Masaaki Inaba, Yoshiki Nishizawa |
Journal | Metabolism: clinical and experimental
(Metabolism)
Vol. 52
Issue 7
Pg. 895-9
(Jul 2003)
ISSN: 0026-0495 [Print] United States |
PMID | 12870167
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Glycated Hemoglobin A
- Insulin
- Glycogen Synthase
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Topics |
- Adult
- Alleles
- Blood Glucose
(analysis)
- Body Mass Index
- Diabetes Mellitus, Type 2
(genetics)
- Female
- Gene Frequency
- Glucose Clamp Technique
- Glycated Hemoglobin
(analysis)
- Glycogen Synthase
(genetics)
- Humans
- Insulin
(blood)
- Insulin Resistance
(genetics)
- Male
- Middle Aged
- Muscle, Skeletal
(enzymology)
- Polymorphism, Genetic
- Regression Analysis
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