Abstract | OBJECTIVE: METHODS: The cardiac myocytes were isolated from neonatal SD rats and cultured in vitro for 72 h. Apoptosis of the cultured cells was induced by hypoxia-reoxygenation and advanced glycation end products (AGEs) respectively, and the effects of flavone extracted from the leaves of Diospyros kaki on the cell apoptosis was observed by measuring the apoptotic rates using flow cytometry. RESULTS: Apoptosis of neonatal rat cardiac myocytes was induced by hypoxia-reoxygenation in a time-dependent manner (6.05% +/-0.46% vs 12.45% +/-1.66%, P< 0.05), and flavone treatment of the cells significantly lowered the apoptotic rates. AGEs alone also induced apoptosis of neonatal rat cardiac myocytes (11.03+/-0.39 vs 6.25+/-0.48, P< 0.05), which was inhibited by flavone (8.40+/-0.47 vs 11.03+/-0.39, P < 0.05). CONCLUSION:
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Authors | Ping Ouyang, Wei-jJian Bei, Wen-yan Lai, Ding-li Xu, Wen-lie Peng |
Journal | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
(Di Yi Jun Yi Da Xue Xue Bao)
Vol. 23
Issue 7
Pg. 680-2
(Jul 2003)
ISSN: 1000-2588 [Print] China |
PMID | 12865219
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Flavonoids
- Glycation End Products, Advanced
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Topics |
- Animals
- Apoptosis
(drug effects)
- Cell Hypoxia
- Diospyros
(chemistry)
- Female
- Flavonoids
(pharmacology)
- Glycation End Products, Advanced
(toxicity)
- Male
- Myocytes, Cardiac
(drug effects, pathology)
- Plant Leaves
(chemistry)
- Rats
- Rats, Sprague-Dawley
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