HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Improved diagnosis of chronic hepatitis C virus infection by detection of antibody to multiple epitopes: confirmation by antibody to synthetic oligopeptides.

Abstract
Serum samples from 226 patients covering a wide spectrum of liver disease were tested for antibodies to hepatitis C virus (HCV) using both first and second generation enzyme linked immunosorbent assays. Selected sera were also tested by peptide immunoassays, by the four-antigen recombinant immunoblot assay (RIBA II), and for viral genome by the polymerase chain reaction. Antibody to c100-3 was detected in 61% of patients with chronic non-A, non-B (NANB) hepatitis and/or 46.5% with presumed NANB-related cirrhosis by the first generation test. These figures increased to 77% and 58% when antibodies to recombinant structural and non-structural HCV antigens were sought by the second generation assay. Supplemental testing against peptide Sp75 and Sp65/sp67 confirmed that reactivity of sera by second generation assays was due to antibodies to the additional structural and non-structural antigens. Samples negative by the first generation assay were not confirmed by the supplemental assay using peptides Sp75 and Sp65/Sp67. HCV RNA was detected in 60% of the anti-HCV positive sera tested, most of which were also RIBA II positive. Our findings confirm that the introduction of the structural and non-structural antigens, especially the putative nucleocapsid protein, improves sensitivity of detection of antibodies to HCV, and facilitates diagnosis in patients with "cryptogenic" chronic hepatitis.
AuthorsD Brown, L Powell, A Morris, S Rassam, S Sherlock, N McIntyre, A J Zuckerman, G M Dusheiko
JournalJournal of medical virology (J Med Virol) Vol. 38 Issue 3 Pg. 167-71 (Nov 1992) ISSN: 0146-6615 [Print] United States
PMID1283751 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Antigens, Viral
  • C100 protein, hepatitis C virus
  • DNA, Single-Stranded
  • Epitopes
  • Hepatitis Antibodies
  • Hepatitis C Antibodies
  • Oligopeptides
  • RNA, Viral
  • Recombinant Proteins
  • Viral Nonstructural Proteins
Topics
  • Antigens, Viral (immunology)
  • Base Sequence
  • Chronic Disease
  • DNA, Single-Stranded (chemistry)
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes (immunology)
  • Hepacivirus (genetics, immunology)
  • Hepatitis Antibodies (blood)
  • Hepatitis C (diagnosis)
  • Hepatitis C Antibodies
  • Humans
  • Immunoblotting
  • Molecular Sequence Data
  • Oligopeptides (immunology)
  • Polymerase Chain Reaction
  • RNA, Viral (blood)
  • Recombinant Proteins (immunology)
  • Viral Nonstructural Proteins (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: