[Construction of eukaryotic expression vector for rat myogenin gene].

Abstract

OBJECTIVE:

To construct eukaryotic expression vector of rat myogenin gene for further study on its functions in skeletal muscle denervated atrophy and repair.

METHODS:

The cloning vectors (containing full length of myogenin cDNA and two restriction sites: Hind III and Xho I) were first cut by two restriction endonuclease: Hind III and Xho I, and the same as the eukaryotic expression vector; then, the myogenin cDNA and the digested vector were ligated by T4 DNA ligase, and recombinant eukaryotic expression vector was formed. Its length was certificated by agarose gel electrophoresis analysis, digestion with Hind III and Xho I, PCR; and the rightness of the myogenin cDNA sequence was confirmed by sequencing.

RESULTS:

The results of agarose gel electrophoresis analysis, digestion, and PCR confirmed the right length of inserted DNA, which was the same as the myogenin cDNA, and the sequencing result of pcDNA3-myogenin was identical with the reported.

CONCLUSION:

pcDNA3-myogenin a eukaryotic expression vector, is successfully constructed.

• Authors: Hao Jiang, Jian-guang Xu, Yu-dong Gu, Shao-nan Hu, Ji-feng Li
• Journal: Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery (Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi) Vol. 17 Issue 3 Pg. 227-9 (May 2003) ISSN: 1002-1892 [Print] China
• PMID: 12822357 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Myog protein, rat
• Myogenin

Topics

• Animals
• Cells, Cultured
• DNA, Complementary (genetics)
• Eukaryotic Cells (metabolism)
• Gene Expression
• Genetic Vectors
• Myogenin (biosynthesis, genetics)
• Rats