Abstract |
The occurrence of epithelial membrane antigen (EMA), low molecular weight cytokeratin (CK) and erbB-2 oncoprotein was investigated in twenty-eight cases of Paget's disease of the nipple (PD) to determine their diagnostic usefulness. The ABC technique with monoclonal antibodies was used on formalin-fixed, paraffin-embedded tissue. The Paget's cells showed positive immunoreactivity for all three antigens investigated in a high percentage of PD. Immunoreactivity for CK and erbB-2 oncoprotein was restricted to the Paget's cells, whereas EMA in some cases also stained the adjacent keratinocytes. Since CK and/or erbB-2 oncoprotein occurred in 93% of the cases, we conclude that demonstration of both antigens is useful in the diagnosis of PD. ErbB-2 oncoprotein was also found to be expressed in a high percentage of the underlying intraductal and invasive carcinomas.
|
Authors | T Haerslev, G Krag Jacobsen |
Journal | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
(APMIS)
Vol. 100
Issue 11
Pg. 1041-7
(Nov 1992)
ISSN: 0903-4641 [Print] Denmark |
PMID | 1282021
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Membrane Glycoproteins
- Mucin-1
- Proto-Oncogene Proteins
- Keratins
- Receptor, ErbB-2
|
Topics |
- Carcinoma, Intraductal, Noninfiltrating
(pathology)
- Humans
- Immunoenzyme Techniques
- Keratins
(metabolism)
- Membrane Glycoproteins
(metabolism)
- Mucin-1
- Nipples
(metabolism)
- Paget's Disease, Mammary
(metabolism)
- Proto-Oncogene Proteins
(metabolism)
- Receptor, ErbB-2
|