Correlation of haemagglutinin-neuraminidase and fusion protein content with protective antibody response after immunisation with inactivated Newcastle disease vaccines.

Abstract

The correlation between the antigen content of inactivated Newcastle disease (ND) oil emulsion-vaccines and the serological response after immunisation was studied. The haemagglutinin-neuraminidase (HN) and fusion (F) proteins of Newcastle disease virus (NDV) were quantified in 33 inactivated oil-adjuvanted ND vaccines using isopropylmyristate (IPM)-extraction and antigen ELISAs. These commercial vaccines differed in NDV-vaccine strain, the method applied to inactivate the vaccine virus, the vaccine valence and the composition of the oil emulsions. Large differences, up to 100-fold, in the antigen quantities present in different vaccines were found. The NDV-HN content and the NDV-F content of the vaccines both highly correlated with the haemagglutination-inhibiting (HI) antibody titres after immunisation. These correlation's were found over a 10,000-fold range of applied antigen. The antigen content of the oil emulsion-vaccines also highly correlated with virus neutralising antibody titres. The presence of serum HI antibody titres in individual specific pathogens free (SPF)-chickens after immunisation with inactivated ND vaccines was highly indicative for clinical protection against challenge with the virulent NDV-Herts strain. Our results are the first to demonstrate that the protective serological response after immunisation highly correlates with the antigen content of oil-adjuvanted vaccines.

• Authors: R A Maas, M Komen, M van Diepen, H L Oei, I J T M Claassen
• Journal: Vaccine (Vaccine) Vol. 21 Issue 23 Pg. 3137-42 (Jul 04 2003) ISSN: 0264-410X [Print] Netherlands
• PMID: 12804840 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Viral
• Antigens, Viral
• Emulsions
• Hemagglutinins
• Oils
• Viral Fusion Proteins
• Viral Vaccines
• Neuraminidase

Topics

• Adjuvants, Immunologic
• Animals
• Antibodies, Viral (analysis, biosynthesis)
• Antigens, Viral (analysis, immunology)
• Chickens
• Dose-Response Relationship, Immunologic
• Emulsions
• Enzyme-Linked Immunosorbent Assay
• Hemagglutinins (biosynthesis)
• Immunization
• Immunoenzyme Techniques
• Neuraminidase (biosynthesis)
• Neutralization Tests
• Newcastle Disease (immunology, prevention & control)
• Newcastle disease virus (immunology)
• Oils
• Poultry Diseases (immunology, prevention & control)
• Viral Fusion Proteins (biosynthesis, genetics)
• Viral Vaccines (immunology)