Signaling pathway for aloe-emodin-induced apoptosis in human H460 lung nonsmall carcinoma cell.

Abstract

Aloe-emodin (1,8-dihydroxy-3-(hydroxymethyl)-anthraquinone) is an active component from the root and rhizome of Rheum palmatum that has been reported to exhibit antitumor effects through an unknown mechanism. Our study investigated the mechanisms of aloe-emodin-induced cell death in the human lung nonsmall cell carcinoma cell line H460. Aloe-emodin (40 microM)-induced apoptosis of H460 cells involves modulation of cAMP-dependent protein kinase, protein kinase C, Bcl-2, caspase-3 and p38 protein expression. The relationship of various signals involved in cell death, such as cAMP-dependent protein kinase, protein kinase C, Bcl-2, caspase-3 and p38, has been investigated in the regulation of apoptotic cell death of aloe-emodin. We demonstrated that the expression of p38 is an important determinant of apoptotic death induced by aloe-emodin.

• Authors: Feng-Tsgh Yeh, Chun-Hsiung Wu, Hong-Zin Lee
• Journal: International journal of cancer (Int J Cancer) Vol. 106 Issue 1 Pg. 26-33 (Aug 10 2003) ISSN: 0020-7136 [Print] United States
• PMID: 12794753 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2003 Wiley-Liss, Inc.

Chemical References

• Alkaloids
• Anthraquinones
• Benzophenanthridines
• Enzyme Inhibitors
• Imidazoles
• Phenanthridines
• Proto-Oncogene Proteins c-bcl-2
• Pyridines
• Xanthines
• Colforsin
• methylxanthine
• aloe emodin anthrone
• aloe emodin
• chelerythrine
• Cyclic AMP-Dependent Protein Kinases
• Protein Kinase C
• Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases
• CASP3 protein, human
• Caspase 3
• Caspases
• Emodin
• 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole

Topics

• Alkaloids
• Anthraquinones
• Apoptosis
• Benzophenanthridines
• Blotting, Western
• Carcinoma, Non-Small-Cell Lung (metabolism)
• Caspase 3
• Caspases (metabolism)
• Cell Death
• Cell Survival
• Colforsin (pharmacology)
• Cyclic AMP-Dependent Protein Kinases (metabolism)
• Emodin (analogs & derivatives, pharmacology)
• Enzyme Inhibitors (pharmacology)
• Humans
• Imidazoles (pharmacology)
• Lung Neoplasms (metabolism)
• Mitogen-Activated Protein Kinases (metabolism)
• Phenanthridines (pharmacology)
• Phosphorylation
• Protein Kinase C (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Pyridines (pharmacology)
• Signal Transduction
• Time Factors
• Tumor Cells, Cultured
• Xanthines (pharmacology)
• p38 Mitogen-Activated Protein Kinases