HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits proliferation of human prostate cancer cells by causing G2/M arrest and inducing apoptosis.

Abstract
Dietary isothiocyanates (ITCs) are highly effective in affording protection against chemically induced cancers in laboratory animals. In the present study, we demonstrate that allyl isothiocyanate (AITC), a constituent of cruciferous vegetables, significantly inhibits proliferation of cultured PC-3 (androgen-independent) and LNCaP (androgen-dependent) human prostate cancer cells in a dose-dependent manner with an IC(50) of approximately 15-17 micro M. On the other hand, survival of a normal prostate epithelial cell line (PrEC) was minimally affected by AITC even at concentrations that were highly cytotoxic to the prostate cancer cells. Reduced proliferation of PC-3 as well as LNCaP cells in the presence of AITC correlated with accumulation of cells in G(2)/M phase and induction of apoptosis. In contrast, AITC treatment failed to induce apoptosis or cause G(2)/M phase arrest in PrEC cells. A 24 h treatment of PC-3 and LNCaP cells with 20 micro M AITC caused a significant decrease in the levels of proteins that regulate G(2)/M progression, including Cdk1 (32-50% reduction), Cdc25B (44-48% reduction) and Cdc25C (>90% reduction). A significant reduction in the expression of cyclin B1 protein (approximately 45%) was observed only in LNCaP cells. A 24 h exposure of PC-3 and LNCaP cells to an apoptosis-inducing concentration of AITC (20 micro M) resulted in a significant decrease (31-68%) in the levels of anti-apoptotic protein Bcl-2 in both cell lines, and approximately 58% reduction in Bcl-X(L) protein expression in LNCaP cells. In conclusion, it seems reasonable to hypothesize that AITC, and possibly other ITCs, may find use in the treatment of human prostate cancers.
AuthorsDong Xiao, Sanjay K Srivastava, Karen L Lew, Yan Zeng, Pamela Hershberger, Candace S Johnson, Donald L Trump, Shivendra V Singh
JournalCarcinogenesis (Carcinogenesis) Vol. 24 Issue 5 Pg. 891-7 (May 2003) ISSN: 0143-3334 [Print] England
PMID12771033 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • BCL2L1 protein, human
  • CCNB1 protein, human
  • Cell Cycle Proteins
  • Cyclin B
  • Cyclin B1
  • Isothiocyanates
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-X Protein
  • allyl isothiocyanate
  • CDC2 Protein Kinase
  • CDC25B protein, human
  • CDC25C protein, human
  • cdc25 Phosphatases
Topics
  • Apoptosis (drug effects)
  • Blotting, Western
  • CDC2 Protein Kinase (metabolism)
  • Cell Cycle Proteins (metabolism)
  • Cell Division (drug effects)
  • Cyclin B (metabolism)
  • Cyclin B1
  • Epithelial Cells (cytology, metabolism)
  • G2 Phase (drug effects)
  • Humans
  • Isothiocyanates (pharmacology)
  • Male
  • Mitosis (drug effects)
  • Prostate (cytology)
  • Prostatic Neoplasms (metabolism, pathology)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Tumor Cells, Cultured
  • Vegetables (chemistry)
  • bcl-X Protein
  • cdc25 Phosphatases (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: