Abstract | BACKGROUND: METHODS: Pregnant CD-1 mice dosed with 2CdA with or without PK11195 on gestation Day 8 provided fetuses for teratologic evaluation on Day 14 and Day 17; HPLC measured pyridine nucleotides ( NADH/ NAD+) at 1.5 hr, RT-PCR measured mitochondrial 16S rRNA abundance at 3.0 hr, and p53 protein induction was assessed with immunostaining at 4.5 hr postexposure. RESULTS: The mean incidences of malformed fetuses were significantly higher in the 7.5 mg/kg 2CdA treatment group (50.2% malformed) vs. the 2CdA + 4.0 mg/kg PK11195 co-treatment group (4.4% malformed). Malformed fetuses displayed a range of ocular defects that included microphthalmia and keratolenticular dysgenesis (Peters anomaly). No malformations were observed in the control or PK11195 alone groups. PK11195 also protected litters from increased resorption rates and fetal weight reduction. It did not rescue early effects on NADH balance (1.5 hr) or 16S rRNA expression (3.0 hr); however, the p53 response (4.5 hr) was downgraded in 2CdA + PK11195 embryos vs. 2CdA alone. By delaying the administration of PK11195 in 1.5 hr intervals it was determined that the window for protection closed between 4.5 to 6.0 hr after 2CdA. CONCLUSIONS:
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Authors | Jeffrey H Charlap, Ronald J Donahue, Thomas B Knudsen |
Journal | Birth defects research. Part A, Clinical and molecular teratology
(Birth Defects Res A Clin Mol Teratol)
Vol. 67
Issue 2
Pg. 108-15
(Feb 2003)
ISSN: 1542-0752 [Print] United States |
PMID | 12769506
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Fetal Proteins
- Isoquinolines
- RNA, Ribosomal, 16S
- Receptors, GABA-A
- Teratogens
- Tumor Suppressor Protein p53
- NAD
- 2-chloro-2',3'-dideoxyadenosine
- Dideoxyadenosine
- PK 11195
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Topics |
- Animals
- Dideoxyadenosine
(administration & dosage, analogs & derivatives, antagonists & inhibitors, toxicity)
- Drug Evaluation, Preclinical
- Eye Abnormalities
(chemically induced, prevention & control)
- Female
- Fetal Proteins
(biosynthesis, genetics)
- Fetal Resorption
(chemically induced, prevention & control)
- Fetus
(drug effects)
- Gene Expression Regulation, Developmental
(drug effects)
- Genes, p53
- Gestational Age
- Isoquinolines
(pharmacology, therapeutic use)
- Mice
- Microphthalmos
(chemically induced, prevention & control)
- Mitochondria
(drug effects, metabolism)
- NAD
(metabolism)
- Pregnancy
- RNA, Ribosomal, 16S
(biosynthesis)
- Receptors, GABA-A
(drug effects)
- Teratogens
(toxicity)
- Tumor Suppressor Protein p53
(biosynthesis, physiology)
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