Abstract | OBJECTIVE: METHOD: SD rat pancreatic islet cells were isolated and cultured in monolayer in vitro followed by incubation with stepwise diluted palmitic acid (0, 0.125, 0.25 mmol/L and 0.5 mmol/L respectively), and the insulin concentrations in the culture medium were determined by radio immunological methods. Morphological observation with a fluorescence microscope was conducted after double staining of the cells with PI/ Hoechst 33342. RESULTS: The glucose-stimulated insulin secretion was inhibited by palmitic acid at the concentration of 0.25 and 0.5 mmol/L, which also induced obvious cell apoptosis. CONCLUSION:
Palmitic acid is capable of inducing islet cell apoptosis in a dose-dependent manner.
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Authors | Yi-zhen Jiang, Yao-ming Xue, Yan Deng, Zi-ying Zhu |
Journal | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
(Di Yi Jun Yi Da Xue Xue Bao)
Vol. 23
Issue 5
Pg. 449-51
(May 2003)
ISSN: 1000-2588 [Print] China |
PMID | 12754126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Apoptosis
(drug effects)
- Cells, Cultured
- Diabetes Mellitus, Type 2
(etiology)
- Dose-Response Relationship, Drug
- Insulin
(metabolism)
- Insulin Secretion
- Islets of Langerhans
(drug effects, pathology)
- Necrosis
- Palmitic Acid
(toxicity)
- Rats
- Rats, Sprague-Dawley
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