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[Apoptosis-inducing effect of palmitic acids on rat pancreatic islet cells in primary culture: a preliminary study].

AbstractOBJECTIVE:
To investigate the in vitro apoptosis-inducing effect of palmitic acid on pancreatic islet cells in primary culture, thereby to understand the role of palmitic acid in the pathogenesis of type 2 diabetes mellitus.
METHOD:
SD rat pancreatic islet cells were isolated and cultured in monolayer in vitro followed by incubation with stepwise diluted palmitic acid (0, 0.125, 0.25 mmol/L and 0.5 mmol/L respectively), and the insulin concentrations in the culture medium were determined by radio immunological methods. Morphological observation with a fluorescence microscope was conducted after double staining of the cells with PI/Hoechst 33342.
RESULTS:
The glucose-stimulated insulin secretion was inhibited by palmitic acid at the concentration of 0.25 and 0.5 mmol/L, which also induced obvious cell apoptosis.
CONCLUSION:
Palmitic acid is capable of inducing islet cell apoptosis in a dose-dependent manner.
AuthorsYi-zhen Jiang, Yao-ming Xue, Yan Deng, Zi-ying Zhu
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA (Di Yi Jun Yi Da Xue Xue Bao) Vol. 23 Issue 5 Pg. 449-51 (May 2003) ISSN: 1000-2588 [Print] China
PMID12754126 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Insulin
  • Palmitic Acid
Topics
  • Animals
  • Apoptosis (drug effects)
  • Cells, Cultured
  • Diabetes Mellitus, Type 2 (etiology)
  • Dose-Response Relationship, Drug
  • Insulin (metabolism)
  • Insulin Secretion
  • Islets of Langerhans (drug effects, pathology)
  • Necrosis
  • Palmitic Acid (toxicity)
  • Rats
  • Rats, Sprague-Dawley

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