Phenotypic and genotypic differentiation of Campylobacter spp. isolated from Austrian broiler farms: a comparison.

Abstract

An identification scheme based on restriction fragment length polymorphism of polymerase chain reaction products (PCR-RFLP) was developed to differentiate isolates of the genera Campylobacter, Arcobacter and Helicobacter. Based on the 16S rRNA gene of these genera, PCR amplified a 1216-bp fragment. The amplicons were digested with the restriction enzymes RsaI and EcoRV. Additional differentiation was obtained using a PCR-assay based on the hippuricase gene. Genotyping was performed on several reference strains from the National Collection of Typing Culture (NCTC), London, and on 130 field isolates. In parallel, a phenotypic differentiation was performed, in order to compare the results. In 119 cases (91.5%) the results obtained from the genotypic characterization were concordant with those from phenotypic testing. Co-infections with Campylobacter jejuni and Campylobacter coli in two samples and seven hippurate-negative C. jejuni-strains were identified by the genotypic method. Furthermore, PCR-RFLP assays identified an atypical isolate as Campylobacter fetus/hyointestinalis.

• Authors: V Jauk, C Neubauer, W Szölgyényi, L Vasicek
• Journal: Avian pathology : journal of the W.V.P.A (Avian Pathol) Vol. 32 Issue 1 Pg. 33-7 (Feb 2003) ISSN: 0307-9457 [Print] England
• PMID: 12745378 (Publication Type: Comparative Study, Journal Article)

Chemical References

• RNA, Ribosomal, 16S

Topics

• Animals
• Austria (epidemiology)
• Campylobacter (genetics, isolation & purification)
• Campylobacter Infections (epidemiology, microbiology, veterinary)
• Chickens
• Genotype
• Phenotype
• Polymorphism, Restriction Fragment Length
• Poultry Diseases (epidemiology, microbiology)
• RNA, Ribosomal, 16S (genetics)