Abstract | BACKGROUND & AIMS: Rho proteins are involved in the regulation of several cellular functions. Data from in vitro studies suggest that RhoA could be involved in the inflammatory response. We investigated the role of RhoA and its downstream effector Rho kinase in intestinal inflammation. METHODS: Activation of RhoA was assessed by pull-down assays. A specific inhibitor of Rho kinase, Y-27632, was used to examine the role of Rho kinase in inflammatory response in vivo and in vitro by molecular biology and by immunological and biochemical approaches. RESULTS: CONCLUSIONS:
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Authors | Jean-Pierre Segain, Diane Raingeard de la Blétière, Vincent Sauzeau, Arnaud Bourreille, Gréory Hilaret, Chrystelle Cario-Toumaniantz, Pierre Pacaud, Jean-Paul Galmiche, Gervaise Loirand |
Journal | Gastroenterology
(Gastroenterology)
Vol. 124
Issue 5
Pg. 1180-7
(May 2003)
ISSN: 0016-5085 [Print] United States |
PMID | 12730857
(Publication Type: Clinical Trial, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amides
- Cytokines
- Enzyme Inhibitors
- Intracellular Signaling Peptides and Proteins
- NF-kappa B
- Pyridines
- Y 27632
- Trinitrobenzenesulfonic Acid
- Protein Serine-Threonine Kinases
- rho-Associated Kinases
- CHUK protein, human
- I-kappa B Kinase
- IKBKB protein, human
- IKBKE protein, human
- rhoA GTP-Binding Protein
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Topics |
- Adolescent
- Adult
- Aged
- Amides
(administration & dosage)
- Animals
- Cells, Cultured
- Colitis
(drug therapy, immunology, metabolism)
- Crohn Disease
(drug therapy, immunology, metabolism)
- Cytokines
(metabolism)
- Enzyme Inhibitors
(administration & dosage)
- Female
- Humans
- I-kappa B Kinase
- Intestinal Mucosa
(enzymology, immunology)
- Intracellular Signaling Peptides and Proteins
- Leukocytes, Mononuclear
(cytology, immunology)
- Male
- Middle Aged
- NF-kappa B
(antagonists & inhibitors)
- Prospective Studies
- Protein Serine-Threonine Kinases
(antagonists & inhibitors, metabolism)
- Pyridines
(administration & dosage)
- Rats
- Rats, Sprague-Dawley
- Trinitrobenzenesulfonic Acid
- rho-Associated Kinases
- rhoA GTP-Binding Protein
(metabolism)
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