Hypermutation in human B cells in vivo and in vitro.

Abstract

We develop our previous observation that a subpopulation of circulating memory IgM(+)IgD(+)CD27(+)B cells belongs to a separate pathway of differentiation in humans. This subpopulation, which represents 5-25% of peripheral B cells, is also present in spleen in the same proportion and displays a marginal-zone-like B cell phenotype. In addition, we describe a short-time in vitro induction model for somatic hypermutation by using the BL2 Burkitt's lymphoma cell line stimulated by a combination of antibodies directed against different surface receptors. This short-time assay allows us to show that mutations are stably introduced in one DNA strand of the BL2 VH gene in the G1 phase of the cell cycle.

• Authors: Sandra Weller, Ahmad Faili, Said Aoufouchi, Quetin Guéranger, Moritz Braun, Claude-Agnès Reynaud, Jean-Claude Weill
• Journal: Annals of the New York Academy of Sciences (Ann N Y Acad Sci) Vol. 987 Pg. 158-65 (Apr 2003) ISSN: 0077-8923 [Print] United States
• PMID: 12727635 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)

Chemical References

• Tumor Necrosis Factor Receptor Superfamily, Member 7

Topics

• B-Lymphocytes (immunology, metabolism)
• Humans
• In Vitro Techniques
• Mutation
• Tumor Necrosis Factor Receptor Superfamily, Member 7 (immunology)