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Nitric oxide and HSV vaginal infection in BALB/c mice.

Abstract
Here we study the role of nitric oxide in the vaginal infection of Balb/c mice with herpes simplex virus type 2. Inducible nitric oxide synthase (iNOS) mRNA was detected by RT-PCR in vaginal tissue and inguinal lymph nodes early postinfection. iNOS was also found to be activated in cells recovered from vaginal washings of infected animals. Animals treated with aminoguanidine (AG), an iNOS inhibitor, showed a dose-dependent increase in vaginal pathology after viral infection compared to controls. Viral titers in vaginal washings and vaginas were higher in AG-treated mice. Treated animals presented higher PMN counts in vaginal washings compared to controls. Histopathology studies revealed a profound inflammatory exudate in vaginal tissue of treated animals. Finally, RT-PCR analysis showed increased expression of the chemokines MIP-2 and RANTES in vaginal tissue and inguinal lymph nodes of these animals.
AuthorsFabián Benencia, Gisela Gamba, Hernan Cavalieri, Maria Cecilia Courreges, Ruben Benedetti, Soledad Maria Villamil, Ernesto Jorge Massouh
JournalVirology (Virology) Vol. 309 Issue 1 Pg. 75-84 (Apr 25 2003) ISSN: 0042-6822 [Print] United States
PMID12726728 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Chemokine CCL5
  • Chemokine CXCL2
  • Chemokines
  • Cxcl2 protein, mouse
  • DNA Primers
  • Nitric Oxide Donors
  • RNA, Messenger
  • Nitric Oxide
  • S-Nitroso-N-Acetylpenicillamine
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
Topics
  • Animals
  • Base Sequence
  • Cell Division (drug effects)
  • Chemokine CCL5 (genetics)
  • Chemokine CXCL2
  • Chemokines (genetics)
  • Chlorocebus aethiops
  • DNA Primers
  • Female
  • Herpes Genitalis (immunology, physiopathology)
  • Herpesvirus 2, Human (pathogenicity, physiology)
  • Lymph Nodes (enzymology, virology)
  • Mice
  • Mice, Inbred BALB C
  • Nitric Oxide (physiology)
  • Nitric Oxide Donors (pharmacology)
  • Nitric Oxide Synthase (genetics)
  • Nitric Oxide Synthase Type II
  • RNA, Messenger (genetics)
  • Reverse Transcriptase Polymerase Chain Reaction
  • S-Nitroso-N-Acetylpenicillamine (pharmacology)
  • Vagina (enzymology, virology)
  • Vero Cells
  • Virus Replication

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