Measurement of soluble fibrin monomer-fibrinogen complex in plasmas derived from patients with various underlying clinical situations.

Abstract

We previously reported a monoclonal antibody named IF-43 that specifically recognizes thrombin-modified fibrinogen (desAA- and desAABB- fibrin monomer) bound with fibrinogen or other D(1) domain-containing plasmic fragments such as fragments X,Y, and D(1), but not intact fibrinogen or cross-linked fibrin degradation products (XDP). Here, we tentatively named such complexes, soluble fibrin monomer (FM) -fibrinogen complex. By utilizing IF-43, we have developed a kit to measure soluble FM-fibrinogen complex and compared the profiles with those of two established molecular markers for thrombo-embolic disorders: i.e. the thrombin-antithrombin complex (TAT) and the D-dimer in plasma of patients who underwent surgery without any thrombo-embolic complications. The result indicated that soluble FM-fibrinogen complex is a distinct entity from the two established molecular markers. We have also attempted to observe their profiles in patients with the disseminated intravascular coagulation syndrome (DIC). Although the pro-files of soluble FM-fibrinogen complex in individual patients appeared to vary from one patient to the other, the plasma level of soluble FM-fibrinogen complex was found to be increased at the initial phase of disseminated intravascular coagulation syndrome. Thus, the soluble FM-fibrinogen complex may serve as an independent molecular marker for the detection of thrombin generation and the diagnosis of thrombosis. The soluble FM-fibrinogen complex may also serve as a risk factor for thrombosis, because it may precipitate as insoluble complexes beyond its threshold in plasma, or when it is modified by thrombin.

• Authors: Kunihiko Nakahara, Yumiko Kazahaya, Yuichi Shintani, Kensuke Yamazumi, Yutaka Eguchi, Shin Koga, Hideo Wada, Michio Matsuda
• Journal: Thrombosis and haemostasis (Thromb Haemost) Vol. 89 Issue 5 Pg. 832-6 (May 2003) ISSN: 0340-6245 [Print] Germany
• PMID: 12719780 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Fibrin Fibrinogen Degradation Products
• antithrombin III-protease complex
• fibrin fragment D
• Antithrombin III
• Fibrin
• Fibrinogen
• Peptide Hydrolases

Topics

• Antithrombin III
• Biomarkers (blood)
• Disseminated Intravascular Coagulation (blood)
• Fibrin (analysis, metabolism)
• Fibrin Fibrinogen Degradation Products (analysis)
• Fibrinogen (analysis, metabolism)
• Humans
• Latex Fixation Tests
• Peptide Hydrolases (blood)
• Plasma (chemistry)
• Protein Binding
• Risk Factors
• Solubility
• Thrombosis (diagnosis)