Protection of interferon-gamma knockout mice against Listeria monocytogenes challenge following intramuscular immunization with DNA vaccines encoding listeriolysin O.

Abstract	In this study we evaluated the efficacy of DNA vaccination of IFN-gamma knockout (GKO) mice against Listeria monocytogenes, as these immunodeficient mice are highly susceptible to infection with low numbers of this intracellular bacterial pathogen. Following intramuscular immunization of BALB/c GKO mice with plasmid DNA constructs encoding recombinant forms of the L. monocytogenes hemolysin, listeriolysin O (LLO), we detected the in vivo induction of a LLO(91-99) peptide-specific, protective immune CTL response equivalent to that observed following similar DNA vaccination of normal BALB/c mice. The observed protection represented greatly enhanced immunity for the GKO host, suggesting that DNA vaccination may provide a useful vaccine alternative for certain immunocompromised host populations.
Authors	Ronald A Barry, H G Archie Bouwer, Tina R Clark, Kenneth A Cornell, David J Hinrichs
Journal	Vaccine (Vaccine) Vol. 21 Issue 17-18 Pg. 2122-32 (May 16 2003) ISSN: 0264-410X [Print] Netherlands
PMID	12706703 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Bacterial Proteins Bacterial Toxins Bacterial Vaccines Heat-Shock Proteins Hemolysin Proteins Peptide Fragments Vaccines, DNA Interferon-gamma hlyA protein, Listeria monocytogenes
Topics	Amino Acid Sequence Animals Bacterial Proteins (immunology) Bacterial Toxins Bacterial Vaccines (administration & dosage, therapeutic use) Female Heat-Shock Proteins (immunology) Hemolysin Proteins Injections, Intramuscular Interferon-gamma (deficiency, genetics, immunology) Listeria monocytogenes (immunology) Listeriosis (immunology) Mice Mice, Inbred BALB C Mice, Knockout Peptide Fragments (chemistry, immunology) T-Lymphocytes, Cytotoxic (immunology) Vaccines, DNA (administration & dosage, therapeutic use)

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