Immunization with genetic toxoids of the Arcanobacterium pyogenes cholesterol-dependent cytolysin, pyolysin, protects mice against infection.

Abstract

Pyolysin (PLO), a cholesterol-dependent cytolysin expressed by Arcanobacterium pyogenes, is an important host-protective antigen. However, this molecule is toxic and requires inactivation prior to its use as a vaccine. Three genetically toxoided, nonhemolytic PLO molecules, HIS-PLO.F(497), HIS-PLO.Delta P(499), and HIS-PLO.A(522), were found to be nontoxic, and vaccinated mice were protected from infection, indicating the potential of these toxoids as vaccines. Furthermore, in a mouse model of infection, A. pyogenes carrying the F(497) mutation was as attenuated as a PLO-deficient strain, indicating that the cytolytic activity of PLO is important in virulence.

• Authors: B Helen Jost, Hien T Trinh, J Glenn Songer, Stephen J Billington
• Journal: Infection and immunity (Infect Immun) Vol. 71 Issue 5 Pg. 2966-9 (May 2003) ISSN: 0019-9567 [Print] United States
• PMID: 12704180 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Bacterial Proteins
• Bacterial Toxins
• Bacterial Vaccines
• Cytotoxins
• Hemolysin Proteins
• Pyolysin, Arcanobacterium pyogenes
• Toxoids
• Cholesterol

Topics

• Actinomycetaceae (immunology, pathogenicity)
• Animals
• Bacterial Proteins
• Bacterial Toxins
• Bacterial Vaccines (immunology)
• Cholesterol (physiology)
• Cytotoxins (immunology)
• Hemolysin Proteins (immunology)
• Immunization
• Mice
• Mice, Inbred ICR
• Toxoids (immunology)
• Virulence