Proteomic analysis of eIF-5A in lung adenocarcinomas.

Abstract

We examined the eIF-5A protein expression in 93 lung adenocarcinomas and 10 uninvolved lung samples using quantitative two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis with identification by mass spectrometry and 2-D Western blots. The same tissue samples were examined for the eIF-5A mRNA expression using oligonucleotide microarrays, and the cellular localization of the eIF-5A protein was examined using immunohistochemical analysis on tissue arrays. Higher eIF-5A protein expression was present in tumors showing poor differentiation, 12/13(th) codon K-ras mutations, p53 nuclear accumulation, and tumors with positive lymphocytic response. The eIF-5A mRNA was also significantly increased in lung adenocarcinomas compared to normal lung, but the eIF-5A protein expression was not correlated to its mRNA levels indicating that the increase in the eIF-5A protein expression in lung tumors is post-transcriptionally/translationally/post-translationally regulated. Patients having a higher eIF-5A protein expression showed a relatively poorer survival suggesting the use of eIF-5A as prognostic marker in lung adenocarcinoma. Moreover, the investigation on agents that inhibit eIF-5A function is encouraged.

• Authors: Guoan Chen, Tarek G Gharib, Dafydd G Thomas, Chiang-Ching Huang, David E Misek, Rork D Kuick, Thomas J Giordano, Mark D Iannettoni, Mark B Orringer, Samir M Hanash, David G Beer
• Journal: Proteomics (Proteomics) Vol. 3 Issue 4 Pg. 496-504 (Apr 2003) ISSN: 1615-9853 [Print] Germany
• PMID: 12687616 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Interferon-alpha
• Interleukin-6
• Peptide Initiation Factors
• RNA, Messenger
• RNA-Binding Proteins
• Tumor Suppressor Protein p53
• eukaryotic translation initiation factor 5A

Topics

• Adenocarcinoma (metabolism, pathology)
• Blotting, Western
• Cell Differentiation
• Cell Division
• Cell Line, Tumor
• Electrophoresis, Gel, Two-Dimensional
• Gene Expression Regulation, Neoplastic
• Genes, ras (genetics)
• Humans
• Immunohistochemistry
• Interferon-alpha (metabolism)
• Interleukin-6 (pharmacology)
• Lung Neoplasms (metabolism, pathology)
• Mutation
• Oligonucleotide Array Sequence Analysis
• Peptide Initiation Factors (analysis, chemistry, genetics, metabolism)
• Protein Biosynthesis
• Proteomics
• RNA, Messenger (genetics, metabolism)
• RNA-Binding Proteins
• Survival Rate
• Tumor Suppressor Protein p53 (metabolism)