The effect of poly (adenosine diphosphate-ribose) polymerase inhibitors on biochemical changes in testicular ischemia-reperfusion injury.

Abstract

PURPOSE:

Poly (adenosine diphosphate [ADP]-ribose) polymerase inhibitors have been used successfully to decrease ischemia-reperfusion injury in several organ systems. We evaluated the efficacy of poly (ADP-ribose) polymerase inhibitors on biochemical changes in testicular ischemia-reperfusion injury.

MATERIALS AND METHODS:

Adult male Wistar rats were divided into 9 groups of 6 each. One group served to determine baseline values of biochemical parameters, 1 that underwent sham operation served as a control, 1 underwent 2 hours of testicular torsion and 4 hours of detorsion, 2 received pretreatment with vehicle (saline or dimethyl sulfoxide) before detorsion and 4 received pretreatment with the poly (ADP-ribose) polymerase inhibitor nicotinamide, 3-aminobenzamide, 1,5-dihydroxyisoquinoline or 4-amino-1,8-naphthalimide before detorsion. Lipid peroxidation products, nitric oxide content and myeloperoxidase activity, an indicator of neutrophil accumulation, were assessed in testicular and renal tissues.

RESULTS:

Testicular torsion-detorsion caused a significant increase in lipid peroxidation products, nitric oxide content and myeloperoxidase activity in ipsilateral testes (p <0.01) but not in the contralateral testes or kidneys. Animals treated with poly (ADP-ribose) polymerase inhibitors had a significant decrease in these biochemical parameters compared with vehicle treated animals (p <0.01).

CONCLUSIONS:

These data emphasize that poly (ADP-ribose) polymerase may have a role in testicular damage caused by ischemia-reperfusion and the inhibition of poly (ADP-ribose) polymerase may be a novel approach to therapy for ischemia-reperfusion injury of the testis.

• Authors: Murat Bozlu, Gülçin Eskandari, Selahittin Cayan, Bülent Canpolat, Erdem Akbay, Uğur Atik
• Journal: The Journal of urology (J Urol) Vol. 169 Issue 5 Pg. 1870-3 (May 2003) ISSN: 0022-5347 [Print] United States
• PMID: 12686864 (Publication Type: Journal Article)

Chemical References

• Benzamides
• Isoquinolines
• Naphthalimides
• Poly(ADP-ribose) Polymerase Inhibitors
• Quinolones
• 4-amino-1,8-naphthalimide
• Niacinamide
• 1,5-dihydroxyisoquinoline
• 3-aminobenzamide
• 1-Naphthylamine

Topics

• 1-Naphthylamine (analogs & derivatives, therapeutic use)
• Animals
• Benzamides (therapeutic use)
• Isoquinolines (therapeutic use)
• Male
• Naphthalimides
• Niacinamide (therapeutic use)
• Poly(ADP-ribose) Polymerase Inhibitors
• Quinolones (therapeutic use)
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, enzymology, etiology)
• Spermatic Cord Torsion (complications, drug therapy, enzymology)
• Testis (blood supply, drug effects)