Abstract | INTRODUCTION: Suicide gene therapy is a new experimental form of cancer chemotherapy that is currently being evaluated in human trials. AIM: METHODOLOGY: The CD gene was cloned into pAdTrack-CMV-CD, pAdTrack-CMV-CD, and pAdEasy-1, which underwent recombination in bacteria BJ5183. The newly recombinant Ad-CD containing green fluorescent protein was propagated in 293 cells and purified by cesium chloride gradient centrifugation. The human pancreatic carcinoma cell line PaTu8988/SW1990 was infected with this virus, and then 5-fluorocytosine (5-FC) was added; XTT assay was used to estimate relative numbers of viable cells. An in vivo model of pancreatic cancer was established by injecting 1.0 x 10(7) PaTu8988/SW1990 cells subcutaneously in Balb/c nude mice. When tumors were palpable, Ad-CD was injected into each tumor, and 5-FC was administered. The positive clones were selected by endonuclease digestion of the combinations, and the concentration of viral liquids containing the CD gene was 2 x 10(11) pfu/mL. RESULTS: It was found that significant cytotoxic activities were possessed by 5-FC for CD gene-transduced PaTu8988/SW1990 cells, but there was little effect on the nontransduced pancreatic carcinoma cells. The antitumor effect was observed in PaTu8988/SW1990 xenografts from nude mice with in situ CD gene transduction. CONCLUSION: These results indicate that the CD gene mediated by adenovirus has a high level of infectivity and is efficient for gene therapy for pancreatic carcinoma.
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Authors | Xue Pan, Zhao-Shen Li, Guo-Ming Xu, Long Cui, Zhen-Xing Tu |
Journal | Pancreas
(Pancreas)
Vol. 26
Issue 3
Pg. 274-8
(Apr 2003)
ISSN: 1536-4828 [Electronic] United States |
PMID | 12657954
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adenoviridae
(genetics)
- Animals
- Bystander Effect
- Carcinoma
(therapy)
- Cell Line, Tumor
- Cytosine Deaminase
(genetics)
- Genetic Therapy
- Genetic Vectors
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Pancreatic Neoplasms
(therapy)
- Transduction, Genetic
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