Abstract | BACKGROUND: Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms. OBJECTIVES: Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy. METHODS: RESULTS: All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol. CONCLUSIONS: S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.
|
Authors | H Akiyama, T Hamada, W-K Huh, O Yamasaki, T Oono, W Fujimoto, K Iwatsuki |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 148
Issue 3
Pg. 526-32
(Mar 2003)
ISSN: 0007-0963 [Print] England |
PMID | 12653745
(Publication Type: Journal Article)
|
Chemical References |
- Fusidic Acid
- Fibrin
- Acetic Acid
- Hydrochloric Acid
|
Topics |
- Acetic Acid
(pharmacology)
- Adolescent
- Adult
- Aged
- Biofilms
(growth & development)
- Child
- Child, Preschool
- Colony Count, Microbial
- Dermatitis, Atopic
(microbiology)
- Female
- Fibrin
- Fusidic Acid
(pharmacology)
- Glycocalyx
(metabolism)
- Humans
- Hydrochloric Acid
(pharmacology)
- Impetigo
(microbiology)
- Male
- Microscopy, Confocal
(methods)
- Middle Aged
- Pemphigus
(microbiology)
- Staphylococcus aureus
(drug effects, physiology)
|