Mild-to-moderate
vitamin C depletion in weanling guinea-pigs affects
pyridinoline:
deoxypyridinoline (
collagen cross-link) ratios in femur shaft and urine, attributed to impairment of hydroxylation of
collagen lysine. We investigated: (1). whether the picture at two time points is compatible with progressive accumulation of abnormal
collagen; (2). whether any changes are seen in skin, where little
deoxypyridinoline occurs; (3). whether total food restriction has similar effects. Male weanling Dunkin-Hartley guinea-pigs were fed diets containing either 0.5 (
vitamin C-restricted) or 160.0-320.0 (
vitamin C-adequate) mg
vitamin C/d. Two groups receiving the
vitamin C-adequate diet received it ad libitum. Two other groups received the
vitamin C-adequate diet in a restricted amount, limited to that which permitted nearly the same growth rate as in the
vitamin C-restricted groups. Animals were fed for 4 or 8 weeks; urine was collected, and
vitamin C and
collagen indices were measured. In the femur shaft, the
hydroxyproline content per unit weight was unaffected by
vitamin C restriction or by total food restriction.
Deoxypyridinoline was increased and the
pyridinoline:
deoxypyridinoline ratio was decreased in
vitamin C-restricted groups, but not in food-restricted groups. Changes in the value of the ratio were greater after 8 than after 4 weeks. Urine indices mirrored bone indices. In skin, the main effect of
vitamin C restriction was to reduce
hydroxyproline. Here, the cross-link ratios changed less markedly than in bone, and there was less
deoxypyridinoline. We conclude that the picture at two time points is compatible with a progressive accumulation of
pyridinoline-enriched
collagen in
vitamin C-deprived animals, that the picture in skin differs from that of bone and urine, and that cross-link changes are not produced by total food restriction.