Abstract | BACKGROUND: METHODS: Kidneys of OLETF and Long-Evans Tokushima Otsuka rats were obtained at ages 7, 20, 50, and 68 weeks after collecting their blood and urine samples. OPB-9195 had been administered to the rats from age 24 weeks to the end of the experiments. CML in kidneys was detected by using a monoclonal antibody against CML according to an indirect immunofluorescence technique. CML-positive glomerular area was measured using NIH Image software (Research Services Branch of NIMH, Bethesda, MD). Hyalinized and/or sclerotic areas in glomeruli and mesangial and glomerular volume were measured using a point-counting technique. RESULTS: CML-positive area in glomeruli correlated closely not only with urinary albumin excretion (r = 0.912; P = 0.001), but also with volumes of mesangium and hyalinized and/or sclerotic lesions (r = 0.859; P = 0.0019 and r = 0.833; P = 0.0027, respectively). Treatment with OPB-9195 reduced CML-positive area and prevented the increase in mesangial volume, with no significant change in glomerular volume at age 68 weeks. The volume of hyalinized and/or sclerotic lesions also decreased by treatment with OPB-9195 in three of four rats at age 68 weeks. CONCLUSION:
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Authors | Sakurako Nakamura, Tetsuya Tachikawa, Kazuki Tobita, Isao Aoyama, Fumio Takayama, Atsushi Enomoto, Toshimitsu Niwa |
Journal | American journal of kidney diseases : the official journal of the National Kidney Foundation
(Am J Kidney Dis)
Vol. 41
Issue 3 Suppl 1
Pg. S68-71
(Mar 2003)
ISSN: 1523-6838 [Electronic] United States |
PMID | 12612956
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Glycation End Products, Advanced
- OPB 9195
- Thiadiazoles
- Thiazolidines
- N(6)-carboxymethyllysine
- Lysine
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Topics |
- Age Factors
- Animals
- Antibodies, Monoclonal
(metabolism)
- Diabetes Mellitus, Type 2
(physiopathology)
- Diabetic Nephropathies
(pathology, prevention & control)
- Disease Models, Animal
- Fluorescent Antibody Technique, Indirect
(methods)
- Glomerular Mesangium
(chemistry, drug effects, metabolism, physiopathology)
- Glycation End Products, Advanced
(antagonists & inhibitors, metabolism)
- Kidney
(chemistry, drug effects, physiopathology)
- Kidney Glomerulus
(chemistry, drug effects, metabolism, physiopathology)
- Lysine
(analogs & derivatives, immunology, metabolism, physiology)
- Rats
- Rats, Inbred OLETF
- Rats, Inbred Strains
- Thiadiazoles
(administration & dosage, pharmacology, therapeutic use)
- Thiazolidines
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