Abstract | BACKGROUND: METHODS: An intrathecal catheter was surgically inserted into the subarachnoid space for spinal drug administration in Sprague-Dawley rats. A laparotomy was performed for ligation and cannulation of the bile-pancreatic duct. Rats were pretreated intrathecally with artificial cerebrospinal fluid (aCSF), D-APV, MS, or combined administration of D-APV and MS. These treatments were given 30 min before noxious visceral stimulation with bradykinin injected through the bile-pancreatic catheter. Spontaneous behavioral activity tests, including cage crossing, rearing, and hind limb extension, were conducted before and after bradykinin injection into the bile-pancreatic duct to assess visceral nociception. RESULTS: Spinal pretreatment of D-APV or low doses of MS partially reduced visceral pain behaviors in this model. Pretreatments with combinations of low doses of MS (0.05-0.5 microg) and D-APV (1 microg) were maximally effective in returning all spontaneous behavioral activities to baseline. CONCLUSIONS:
|
Authors | Ying Lu, Louis P Vera-Portocarrero, Karin N Westlund |
Journal | Anesthesiology
(Anesthesiology)
Vol. 98
Issue 3
Pg. 734-40
(Mar 2003)
ISSN: 0003-3022 [Print] United States |
PMID | 12606920
(Publication Type: Journal Article)
|
Chemical References |
- Excitatory Amino Acid Antagonists
- Receptors, N-Methyl-D-Aspartate
- 2-Amino-5-phosphonovalerate
- Morphine
- Bradykinin
|
Topics |
- 2-Amino-5-phosphonovalerate
(administration & dosage)
- Animals
- Bradykinin
(pharmacology)
- Disease Models, Animal
- Drug Therapy, Combination
- Excitatory Amino Acid Antagonists
(administration & dosage)
- Male
- Morphine
(administration & dosage)
- Pain
(drug therapy)
- Pancreatitis
(pathology, physiopathology)
- Rats
- Rats, Sprague-Dawley
- Receptors, N-Methyl-D-Aspartate
(antagonists & inhibitors)
|