Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: The sensitivity of the assay was 1:10(6) peripheral WBCs. Four patients with stage IV neuroblastoma and one patient with stage III neuroblastoma were scored positive. The other stage III patient and the other two patients with ganglioneuroma and ganglioneuroblastoma followed by acute lymphoblastic leukemia, respectively, were scored negative. Control bone marrow aspirates were also negative. The TH assay is more sensitive than immunohistochemical detection, and the results of the TH assay corresponded with the results of MYCN amplification. CONCLUSIONS: The described TH assay is specific, sensitive, and semiquantitative and can be used for the detection of neuroblastoma cell involvement in bone marrow and blood at diagnosis and during therapy. Furthermore, the TH assay is a possible prognostic marker for neuroblastoma.
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Authors | Lambert H J Lambooy, Corrie E M Gidding, Lambert P van den Heuvel, Christina A Hulsbergen-van de Kaa, Marjolijn Ligtenberg, Jos P M Bökkerink, Ronney A De Abreu |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 9
Issue 2
Pg. 812-9
(Feb 2003)
ISSN: 1078-0432 [Print] United States |
PMID | 12576454
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- DNA Primers
- RNA, Messenger
- Tyrosine 3-Monooxygenase
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Topics |
- Base Sequence
- Biomarkers, Tumor
- Brain Neoplasms
(diagnosis, genetics)
- DNA Primers
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Genes, myc
- Humans
- Immunohistochemistry
- Neoplasm, Residual
(diagnosis, genetics)
- Neuroblastoma
(diagnosis, genetics)
- Polymerase Chain Reaction
(methods)
- Predictive Value of Tests
- Prognosis
- RNA, Messenger
(genetics)
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Tumor Cells, Cultured
- Tyrosine 3-Monooxygenase
(genetics)
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