Abstract |
The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported previously (H. Hirata et al., J. Clin. Microbiol. 40:1470-1474, 2002). However, the ELISA with the GST/Be82 antigen cross-reacted with Babesia caballi-infected horse sera, despite the high rate of detection of B. equi. These results suggested that GST/Be82 has an antigen in common with B. caballi or antigenicity similar to that of B. caballi. In the present study, we constructed a series of five clones with deletions in the Be82 gene product, each of which was fused with GST, and used them in ELISAs in order to overcome the cross-reactivity seen with B. caballi. One of the deletion clones, a clone with a deletion of the Be82 gene from positions 236 to 381 (Be82/236-381), specifically and sensitively detected B. equi-infected horse sera without cross-reactivity with B. caballi-infected horse sera. Assays with clones from which other gene products were deleted showed decreased sensitivities or remained nonspecific for the detection of B. equi-infected horse sera. These results suggest that the Be82/236-381 gene product is a novel antigen for the diagnosis of B. equi infection in horses.
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Authors | Haruyuki Hirata, Xuenan Xuan, Naoaki Yokoyama, Yoshifumi Nishikawa, Kozo Fujisaki, Naoyoshi Suzuki, Ikuo Igarashi |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 41
Issue 2
Pg. 547-51
(Feb 2003)
ISSN: 0095-1137 [Print] United States |
PMID | 12574244
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Protozoan
- Protozoan Proteins
- Recombinant Fusion Proteins
- Glutathione Transferase
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Topics |
- Amino Acid Sequence
- Animals
- Antigens, Protozoan
(analysis)
- Babesia
(classification, genetics, isolation & purification)
- Enzyme-Linked Immunosorbent Assay
- Gene Deletion
- Glutathione Transferase
(genetics)
- Molecular Sequence Data
- Protozoan Proteins
(genetics)
- Recombinant Fusion Proteins
(analysis)
- Sequence Homology, Amino Acid
- Serologic Tests
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