HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Vitamin E supplementation does not alter azoxymethane-induced colonic aberrant crypt foci formation in young or old mice.

Abstract
Vitamin E, part of the body's primary lipid-soluble defense against free radicals and reactive oxygen molecules, has been suggested to reduce the risk for some cancers. However, the role of vitamin E in the etiology and prevention of colon cancer, especially in the highest risk group, the aged, is not clear. Thus, this study was conducted to elucidate the effect of vitamin E supplementation on susceptibility to colon cancer by examining azoxymethane (AOM)-induced aberrant crypt foci (ACF) formation, a surrogate biomarker of colon cancer. Young (3-4 mo) and old (19-20 mo) C57BL/6JNIA mice were fed either a control diet (30 mg dl-alpha-tocopheryl acetate/kg diet) or a vitamin E-supplemented diet (500 mg dl-alpha-tocopheryl acetate/kg diet) for 16 wk. After 6 wk of dietary supplementation, young and old mice were injected with saline or AOM weekly for 5 wk to receive the same total dose of AOM (2.2 mg) and killed 10 wk after the first AOM injection. Vitamin E supplementation had no effect on the number of AOM-induced ACF in young or old mice. In addition, vitamin E supplementation did not have an effect on splenocyte interferon-gamma, interluekin-6 and tumor necrosis factor-alpha levels, natural killer cell killing activity or colonic cell proliferation in young or old mice. Thus, alpha-tocopherol does not seem to affect the initiation and early promotion stages of AOM-induced colon carcinogenesis in young or old mice. Whether vitamin E supplementation might be effective in reducing AOM-induced colon tumors is unclear.
AuthorsHeekyung Chung, Dayong Wu, Sung Nim Han, Raina Gay, Barry Goldin, Roderick E Bronson, Joel B Mason, Donald E Smith, Simin Nikbin Meydani
JournalThe Journal of nutrition (J Nutr) Vol. 133 Issue 2 Pg. 528-32 (Feb 2003) ISSN: 0022-3166 [Print] United States
PMID12566495 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Antioxidants
  • Carcinogens
  • Interleukin-6
  • Vitamin E
  • Interferon-gamma
  • Azoxymethane
Topics
  • Aging (immunology)
  • Animals
  • Antioxidants (administration & dosage, pharmacology)
  • Azoxymethane (toxicity)
  • Carcinogens (toxicity)
  • Choristoma
  • Colonic Neoplasms (chemically induced, immunology)
  • Interferon-gamma (biosynthesis)
  • Interleukin-6 (biosynthesis)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Vitamin E (administration & dosage, immunology, pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: