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Importance of IgG2c isotype in the immune response to beta-amyloid in amyloid precursor protein/transgenic mice.

Abstract
A careful analysis of the immune response to immunization of amyloid precursor protein/transgenic (APP/Tg) mice with beta-amyloid (Abeta) may provide insights into why a subset of the patients in a clinical trial receiving Abeta-immunotherapy developed encephalomyelitis. Characterization of isotypic immune responses have been reported in different APP/Tg models. In these studies the relative ratios of IgG1 to IgG2a anti-Abeta antibodies has been used as an indirect measure of T helper 1 (Th1) and Th2 types immune responses. However, it has previously been shown that certain strains of mice, C57Bl/6, C57Bl/10, SJL, and NOD, have an IgG2c rather than an IgG2a gene. Since a substantial number of Abeta-immunization studies rely on APP/Tg mice that have at least one parental C57Bl/6 strain, we have investigated whether antibodies specific for IgG2a can be used for characterization of antibody isotypes in APP/Tg2576 mice. Our results suggest that APP/Tg2576 and major histocompatibilty complex-matched parental strains are not expressing IgG2a, producing instead IgG2c anti-Abeta antibodies.
AuthorsIrina Petrushina, Mike Tran, Nadya Sadzikava, Anahit Ghochikyan, Vitaly Vasilevko, Michael G Agadjanyan, David H Cribbs
JournalNeuroscience letters (Neurosci Lett) Vol. 338 Issue 1 Pg. 5-8 (Feb 20 2003) ISSN: 0304-3940 [Print] Ireland
PMID12565127 (Publication Type: Comparative Study, Journal Article)
CopyrightCopyright 2002 Elsevier Science Ireland Ltd.
Chemical References
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor
  • Immunoglobulin G
  • Immunoglobulin Isotypes
Topics
  • Amyloid beta-Peptides (genetics, immunology)
  • Amyloid beta-Protein Precursor (genetics, immunology)
  • Animals
  • Immunoglobulin G (biosynthesis)
  • Immunoglobulin Isotypes (biosynthesis)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Th1 Cells (immunology, metabolism)
  • Th2 Cells (immunology, metabolism)

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