Abstract |
A careful analysis of the immune response to immunization of amyloid precursor protein/transgenic (APP/Tg) mice with beta-amyloid (Abeta) may provide insights into why a subset of the patients in a clinical trial receiving Abeta- immunotherapy developed encephalomyelitis. Characterization of isotypic immune responses have been reported in different APP/Tg models. In these studies the relative ratios of IgG1 to IgG2a anti-Abeta antibodies has been used as an indirect measure of T helper 1 (Th1) and Th2 types immune responses. However, it has previously been shown that certain strains of mice, C57Bl/6, C57Bl/10, SJL, and NOD, have an IgG2c rather than an IgG2a gene. Since a substantial number of Abeta-immunization studies rely on APP/Tg mice that have at least one parental C57Bl/6 strain, we have investigated whether antibodies specific for IgG2a can be used for characterization of antibody isotypes in APP/Tg2576 mice. Our results suggest that APP/Tg2576 and major histocompatibilty complex-matched parental strains are not expressing IgG2a, producing instead IgG2c anti-Abeta antibodies.
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Authors | Irina Petrushina, Mike Tran, Nadya Sadzikava, Anahit Ghochikyan, Vitaly Vasilevko, Michael G Agadjanyan, David H Cribbs |
Journal | Neuroscience letters
(Neurosci Lett)
Vol. 338
Issue 1
Pg. 5-8
(Feb 20 2003)
ISSN: 0304-3940 [Print] Ireland |
PMID | 12565127
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright 2002 Elsevier Science Ireland Ltd. |
Chemical References |
- Amyloid beta-Peptides
- Amyloid beta-Protein Precursor
- Immunoglobulin G
- Immunoglobulin Isotypes
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Topics |
- Amyloid beta-Peptides
(genetics, immunology)
- Amyloid beta-Protein Precursor
(genetics, immunology)
- Animals
- Immunoglobulin G
(biosynthesis)
- Immunoglobulin Isotypes
(biosynthesis)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Transgenic
- Th1 Cells
(immunology, metabolism)
- Th2 Cells
(immunology, metabolism)
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