Abstract | BACKGROUND: Myo- inositol plays a key role in an important intracellular signalling pathway. A deranged myo- inositol metabolism has been associated with neural tube defects. A myo- inositol loading test was performed to investigate the kinetics in healthy women of reproductive age. METHODS: Five healthy non-obese females [mean age (standard deviation: SD) 22.8 (2.2) years] were recruited at the University Medical Center Nijmegen. Blood samples were drawn fasting and at 20, 40, 60, 90, 180 and 270 min after ingestion of 100 mg/kg body weight of myo- inositol. Urine samples were collected before myo- inositol loading and at 180 and 270 min post-loading. Samples were analysed for serum myo-, epi- and scyllo-inositol and glucose concentrations by gas chromatography. Plasma insulin concentrations were determined by radio-immunoassay. Random intercept models were fitted to evaluate the data. RESULTS: The estimated myo- inositol and scyllo-inositol concentrations both reached maximum values at 180 min post-loading, respectively: mean (SD) 101.5 (9.2) micro mol/L and 1.09 (0.11) micro mol/L. The estimated plasma insulin and serum glucose concentrations decreased slightly but significantly during the experiment: P < 0.0001 and P < 0.05, respectively. At 180 and 270 min post-loading, urinary myo- inositol concentrations were increased and urinary glucose concentrations were unchanged. CONCLUSIONS:
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Authors | Pascal M W Groenen, Hans M W M Merkus, Fred C G J Sweep, Ron A Wevers, Fokje S M Janssen, Régine P M Steegers-Theunissen |
Journal | Annals of clinical biochemistry
(Ann Clin Biochem)
Vol. 40
Issue Pt 1
Pg. 79-85
(Jan 2003)
ISSN: 0004-5632 [Print] England |
PMID | 12542915
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adult
- Chromatography, Gas
- Female
- Humans
- Inositol
(blood, pharmacology, urine)
- Insulin
(blood)
- Kinetics
- Models, Biological
- Radioimmunoassay
(methods)
- Time Factors
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