Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: Elevated levels of p125(FAK) expression were demonstrated in both primary colorectal tumors and colorectal liver metastases compared with normal colorectal mucosa. Immunohistochemistry experiments demonstrated equivalent FAK expression in matched samples of colorectal primary tumors and liver metastases. Using real-time PCR in 39 matched samples, FAK mRNA copy number was significantly higher in primary colorectal tumors compared with normal colorectal mucosa. FAK expression was analyzed by both real-time PCR and immunohistochemistry in a separate set of colorectal liver metastases. Immunohistochemistry demonstrated high levels of FAK expression in 89% of samples. Furthermore, FAK mRNA copies in these unmatched liver metastases were significantly higher than the primary tumor FAK mRNA copies. CONCLUSION:
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Authors | Amy L Lark, Chad A Livasy, Benjamin Calvo, Laura Caskey, Dominic T Moore, XiHui Yang, William G Cance |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 9
Issue 1
Pg. 215-22
(Jan 2003)
ISSN: 1078-0432 [Print] United States |
PMID | 12538472
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- RNA, Messenger
- Protein-Tyrosine Kinases
- Focal Adhesion Kinase 1
- Focal Adhesion Protein-Tyrosine Kinases
- PTK2 protein, human
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Topics |
- Blotting, Western
- Carcinoma
(enzymology, pathology)
- Colorectal Neoplasms
(enzymology, pathology)
- Focal Adhesion Kinase 1
- Focal Adhesion Protein-Tyrosine Kinases
- Humans
- Immunohistochemistry
- Liver Neoplasms
(enzymology, secondary)
- Protein-Tyrosine Kinases
(biosynthesis)
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Up-Regulation
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