Abstract | BACKGROUND: OBJECTIVES: METHODS: RESULTS: One hour after PDT, cells with eosinophilic cytoplasm and markedly stained nuclei were found, and vacuolation of some tumour cells was noted in the lower layer of the epidermis. An infiltrate of lymphocytes and neutrophils was observed in the upper layer of the dermis. One day after PDT, all layers of the epidermis exhibited slightly degenerative necrosis, with shadow cell formation and chromatin condensation around the nuclear membrane in the lower layer of the epidermis. Necrosis in all layers of the epidermis and lymphocyte infiltration in the dermis were found 3 days after PDT. Tumour cells had disappeared and regenerative thickening of the epidermis was observed 7 days after PDT. TUNEL staining revealed apoptosis-positive cells in the epidermis in 8 of 11 specimens obtained 1 day after PDT. Activated caspase-3 expression was noted in the lower layer of the epidermis in four of these eight TUNEL-positive specimens. CONCLUSIONS: Results suggested that apoptosis is involved in tumour cell death after PDT in patients with AK, and that it occurs within 1 day after PDT.
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Authors | H Nakaseko, M Kobayashi, Y Akita, Y Tamada, Y Matsumoto |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 148
Issue 1
Pg. 122-7
(Jan 2003)
ISSN: 0007-0963 [Print] England |
PMID | 12534605
(Publication Type: Journal Article)
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Topics |
- Aged
- Aged, 80 and over
- Apoptosis
- Epidermis
(pathology)
- Facial Dermatoses
(drug therapy, pathology)
- Female
- Humans
- Immunoenzyme Techniques
- In Situ Nick-End Labeling
- Keratosis
(drug therapy, pathology)
- Male
- Middle Aged
- Photochemotherapy
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