[Protective effect of tanshinone on injured cultured PC12 cells in vitro].

Abstract

OBJECTIVE:

To investigate the protective effects of tanshinone on ischemia-like injury models.

METHOD:

Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and glutamic acid injury were used to assay the anti-ischemic roles of tanshinone in cultured PC12 cells, by using morphological examination, MTT staining and LDH measurement.

RESULT:

It was found that 3. 125-200 microliters.ml-1 tanshinone possessed obvious protective effects on PC12 cells from six ischemia-like injury models. Tanshinone could increase the number of life cells and decrease LDH activity significantly, particularly in hypoxia and caffeine injured model.

CONCLUSION:

Tanshinone protected PC12 cells from all injury models effectively in vitro. Its action may mainly focus on inhibiting development of the primary period of ischemia injury and calcium overloading injury.

• Authors: L N He, J Yang, Y Jiang, J Wang, C Liu, S B He
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 26 Issue 6 Pg. 413-6 (Jun 2001) ISSN: 1001-5302 [Print] China
• PMID: 12528512 (Publication Type: Journal Article)

Chemical References

• Abietanes
• Free Radical Scavengers
• Neuroprotective Agents
• Phenanthrenes
• tanshinone
• L-Lactate Dehydrogenase

Topics

• Abietanes
• Animals
• Cell Division (drug effects)
• Free Radical Scavengers (pharmacology)
• L-Lactate Dehydrogenase (metabolism)
• Neuroprotective Agents (pharmacology)
• PC12 Cells
• Phenanthrenes (pharmacology)
• Rats