Abstract | BACKGROUND & OBJECTIVE: METHODS: Cell surviving fraction was determined using the trypan blue dye exclusion assay. The expression levels of bcl-2 protein were assayed by immunofluorescence using fluorescein isothiocyanate label. Apoptosis was detected by Giemsa staining and flow cytometry. RESULTS: Treatment with the two AS-ODNs/ Ara-C combination respectively for 48 h significantly inhibited the growth of AL and CLL cells. There was significant difference on AL and CLL cells survival between nonsense oligodeoxynucleotide(NS-ODN)/ Ara-C combination and Ara-C treated cells alone (P < 0.05). It was found that the two AS-ODNs respectively combined with Ara-C could significantly downregulate bcl-2 protein expression (P < 0.05). There was a significant increase in apoptotic rates of AL or CLL cells after treatment with the two AS-ODNs respectively combined with Ara-C as respectively compared with the NS-ODN/ Ara-C combination and Ara-C treated cells alone; and the effect had statistical difference (P < 0.05). Compared to the AS-ODN directed against the translation initiation of bcl-2 mRNA, the AS-ODN directed against the coding region showed stronger effect in the aspects of increasing the sensitivity of AL cells to Ara-C (P < 0.05). CONCLUSIONS: The two novel AS-ODNs of new target point in the translation initiation and the coding region of bcl-2 mRNA could enhance Ara-C induced apoptosis of AL and CLL cells.
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Authors | Xiao-yong Lei, Huan Zhang, Dong-mei He |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 21
Issue 12
Pg. 1301-4
(Dec 2002)
China |
PMID | 12520735
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Antimetabolites, Antineoplastic
- Oligodeoxyribonucleotides, Antisense
- Proto-Oncogene Proteins c-bcl-2
- Cytarabine
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Topics |
- Antimetabolites, Antineoplastic
(pharmacology)
- Apoptosis
- Cytarabine
(pharmacology)
- Drug Interactions
- HL-60 Cells
- Humans
- K562 Cells
- Leukemia
(pathology)
- Oligodeoxyribonucleotides, Antisense
(pharmacology)
- Proto-Oncogene Proteins c-bcl-2
(antagonists & inhibitors, genetics)
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