Cytokine-induced neutrophil chemoattractant 1 (CINC-1) mediates the sympathetic component of inflammatory mechanical hypersensitivitiy in rats.

Abstract

The hyperalgesic effect of cytokine-induced neutrophil chemoattractant 1 (CINC-1/CXCL1) was measured in a model of mechanical hyperalgesia in rats. CINC-1 evoked a dose-dependent mechanical hypersensitivity, which was already significant 2 h after the cytokine injection, peaked 4 h after and decreased thereafter. The local pre-treatment of the rats with the beta-adrenoceptor antagonist, atenolol (25 microg paw-1), but not with the cyclooxygenase inhibitor indomethacin (100 microg paw-1), inhibited (86%) the CINC-1-induced hypersensitivity. Conversely, IL-1beta-evoked hypersensitivity was inhibited (76%) by local pre-treatment of the animals with indomethacin, but not by atenolol. Carrageenin- and TNF-alpha-evoked hypersensitivity were attenuated to about the same extent (50%) by antisera neutralising CINC-1 or IL-1beta. The association of both antisera abolished the hypersensitivity effect of carrageenin and TNF-alpha. In addition, carrageenin, LPS and TNF-alpha were shown to stimulate the production of immunoreactive CINC-1 in the skin of injected paws. These data suggest that CINC-1, released at sites of inflammation, mediates inflammatory hyperalgesia in rats via release of sympathomimetic amines.

• Authors: Berenice B Lorenzetti, Fabiane H Veiga, Claudio A Canetti, Stephen Poole, Fernando Q Cunha, Sérgio H Ferreira
• Journal: European cytokine network (Eur Cytokine Netw) 2002 Oct-Dec Vol. 13 Issue 4 Pg. 456-61 ISSN: 1148-5493 [Print] France
• PMID: 12517731 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CXCL1
• Chemokines
• Chemokines, CXC
• Chemotactic Factors
• Cxcl1 protein, rat
• Inflammation Mediators
• Intercellular Signaling Peptides and Proteins
• Interleukin-1
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Atenolol
• Carrageenan
• Indomethacin

Topics

• Animals
• Atenolol (pharmacology)
• Carrageenan (toxicity)
• Chemokine CXCL1
• Chemokines (antagonists & inhibitors, pharmacology, physiology)
• Chemokines, CXC
• Chemotactic Factors (antagonists & inhibitors, pharmacology, physiology)
• Hyperalgesia (etiology, physiopathology)
• Indomethacin (pharmacology)
• Inflammation Mediators (antagonists & inhibitors, pharmacology, physiology)
• Intercellular Signaling Peptides and Proteins (pharmacology, physiology)
• Interleukin-1 (antagonists & inhibitors, physiology)
• Lipopolysaccharides (toxicity)
• Male
• Nociceptors (drug effects, physiopathology)
• Rats
• Rats, Wistar
• Sympathetic Nervous System (drug effects, physiopathology)
• Tumor Necrosis Factor-alpha (pharmacology)