HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Expression of FKBP12 in benign and malignant vascular endothelium: an immunohistochemical study on conventional sections and tissue microarrays.

Abstract
FKBP12 is a cytosolic FK506 binding protein that interacts with calcineurin and thereby mediates the immunosuppressive effects of FK506. Because initial immunohistochemical staining showed abundant expression of FKBP12 in vascular endothelial cells, we evaluated whether it could serve as a marker for vascular neoplasms. We performed immunohistochemical staining of conventional sections from formalin-fixed, paraffin-embedded tissue from 59 benign and malignant vascular neoplasms using a polyclonal rabbit antiserum against FKBP12. Western blot analysis of tissue from 6 angiosarcomas showed a single band at 12 kD, consistent with the published molecular weight for the FKBP12 protein. Together, CD31, CD34, and FKBP12 identified all 59 vascular neoplasms in this study. Specificity of immunohistochemical staining was assessed on 1,321 tissues represented on 7 tissue microarrays. All proteins were occasionally expressed in non-vascular tissue. Six of 8 vascular neoplasms represented on the arrays stained for FKBP12, as did normal vessels in numerous cores. The polyclonal antiserum shows comparable sensitivity (94.9%) and specificity (96.5%) to CD34 and CD31 and may be a useful additional marker for vascular differentiation. Because we have evaluated a large number of tissues by tissue microarray, we anticipate that our estimate of the specificity of immunostaining for FKBP12 as a marker for vascular endothelium will be accurate. In addition, our findings may explain the toxic effects of FK506 on vascular endothelium of the kidney.
AuthorsJohn P T Higgins, Kelli Montgomery, Lingli Wang, Elizabeth Domanay, Roger A Warnke, James D Brooks, Matt van de Rijn
JournalThe American journal of surgical pathology (Am J Surg Pathol) Vol. 27 Issue 1 Pg. 58-64 (Jan 2003) ISSN: 0147-5185 [Print] United States
PMID12502928 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Biomarkers, Tumor
  • Neoplasm Proteins
  • Tacrolimus Binding Protein 1A
Topics
  • Animals
  • Biomarkers, Tumor (metabolism)
  • Blotting, Western
  • Endothelium, Vascular (metabolism, pathology)
  • Hemangioendothelioma, Epithelioid (blood supply, metabolism, pathology)
  • Hemangioma (blood supply, metabolism, pathology)
  • Hemangiosarcoma (blood supply, metabolism, pathology)
  • Histocytological Preparation Techniques
  • Humans
  • Immunohistochemistry
  • Neoplasm Proteins (metabolism)
  • Neoplasms, Vascular Tissue (blood supply, metabolism, pathology)
  • Rabbits
  • Sarcoma, Kaposi (blood supply, metabolism, pathology)
  • Sensitivity and Specificity
  • Tacrolimus Binding Protein 1A (analysis, biosynthesis)
  • Vascular Neoplasms (blood supply, metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: