Abstract | OBJECTIVE: To evaluate the effect of interleukin-1alpha (IL-1alpha) on the mesenteric circulation, intestinal mucosal integrity, and bacterial translocation in a burn/ endotoxemia chronic porcine model. SUMMARY BACKGROUND DATA: Major burn and sepsis are associated with a high mortality, ischemia/reperfusion injury to the intestine, and an increased rate of bacterial translocation. Pathologic alterations of IL-1 synthesis, degradation, and binding to receptors have been reported. Manipulation of IL-1-mediated effects might be of therapeutic utility. METHODS: Twenty-one female pigs were instrumented with an ultrasonic flow probe on the superior mesenteric artery and a catheter into the superior mesenteric vein. After 5 days, all animals were anesthetized, and 14 received 40% total body surface area third-degree burn. IL-1alpha was administered intravenously at 1,000 ng/kg to seven pigs immediately after burn. Eighteen hours after burn, 100 microg/kg lipopolysaccharide (LPS) was administered intravenously. Systemic and splanchnic hemodynamics were measured and blood samples were drawn for blood gas analysis. Intestinal permeability was assessed every 6 hours by measuring the lactulose/ mannitol (L/M) excretion ratio. At the end of the study (42 hours), tissue samples were harvested for bacteriologic cultures. RESULTS: Mesenteric blood flow was significantly decreased after burn and endotoxin. Administration of IL-1alpha significantly improved mesenteric blood flow postburn and post-LPS. Mesenteric oxygen supply and consumption showed a significant reduction after burn. In contrast, animals treated with IL-1alpha showed an increase in postburn mesenteric oxygen supply and consumption. LPS-induced mesenteric hypoxia was also ameliorated by IL-1alpha treatment. Intestinal permeability, as assessed by the L/M ratio, showed a 7- and 10-fold elevation after thermal injury and LPS, respectively. In contrast, IL-1alpha-treated animals showed an increase of only three- and fourfold in the L/M ratio, respectively. Bacterial translocation was significantly increased in the burn/ endotoxin group. IL-1alpha significantly reduced the rates of bacterial translocation. CONCLUSIONS:
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Authors | Tamer Tadros, Daniel L Traber, John P Heggers, David N Herndon |
Journal | Annals of surgery
(Ann Surg)
Vol. 237
Issue 1
Pg. 101-9
(Jan 2003)
ISSN: 0003-4932 [Print] United States |
PMID | 12496536
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
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Topics |
- Analysis of Variance
- Animals
- Bacterial Translocation
(drug effects)
- Burns
(complications, drug therapy)
- Cell Membrane Permeability
(drug effects)
- Disease Models, Animal
- Female
- Interleukin-1
(pharmacology)
- Intestinal Diseases
(drug therapy)
- Intestinal Mucosa
(drug effects, physiology)
- Ischemia
(drug therapy)
- Oxygen Consumption
- Probability
- Random Allocation
- Reference Values
- Reperfusion Injury
(drug therapy)
- Sensitivity and Specificity
- Sepsis
(complications, drug therapy)
- Splanchnic Circulation
(drug effects)
- Swine, Miniature
- Treatment Outcome
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