IS20I, a specific alphavbeta3 integrin inhibitor, reduces glioma growth in vivo.

Abstract

OBJECTIVE:

The biological features of malignant gliomas include high cell proliferation, extensive local infiltration of tumor cells into normal brain, and marked neovascularization. alphavbeta3 integrin is highly expressed in malignant gliomas and plays a role in glioma growth. This article investigates the in vitro and in vivo effects of a synthetic alphavbeta3 integrin inhibitor called IS20I on human malignant gliomas.

METHODS:

The in vitro effects of IS20I were studied by performing adhesion assays, competition studies, semi-in vivo angiogenic assays, and migration and proliferation assays. For the in vivo experiments, IS20I was administered systemically in nude mouse intracranial and subcutaneous malignant glioma models.

RESULTS:

IS20I reacted selectively to alphavbeta3 integrin in glioma cells and tissues. In vitro, IS20I strongly inhibited angiogenesis and simultaneously exhibited potent antimitotic and antimigratory effects on numerous tumor and endothelial cell lines. In addition, at high concentrations, IS20I induced endothelial and tumor cell apoptosis. In vivo, when IS20I was administered intraperitoneally in subcutaneous and intracranial nude mouse glioma models, it potently reduced malignant glioma growth. Inhibition levels of 76 and 82% were observed at concentrations of 1 and 5 mg/kg, respectively, in the U87 intracranial model. The suppression of tumor growth is associated with a decrease in tumor vascularity, an increase in apoptosis, and a decrease in tumor cell proliferation.

CONCLUSION:

This work expands the understanding of the effects of anti-alphavbeta3 integrin inhibitors on malignant gliomas. In addition to direct proapoptotic and antiangiogenic effects, IS20I inhibits tumor and endothelial cell proliferation and migration, resulting in a potent inhibition of glioma growth in vivo.

• Authors: Lorenzo Bello, Valeria Lucini, Carlo Giussani, Giorgio Carrabba, Mauro Pluderi, Francesco Scaglione, Giustino Tomei, Roberto Villani, Peter McL Black, Andreas Bikfalvi, Rona S Carroll
• Journal: Neurosurgery (Neurosurgery) Vol. 52 Issue 1 Pg. 177-85; discussion 185-6 (Jan 2003) ISSN: 0148-396X [Print] United States
• PMID: 12493116 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• IS20I
• Integrin alphaVbeta3
• Oligopeptides
• Peptides, Cyclic

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Brain Neoplasms (pathology)
• Cell Adhesion (drug effects)
• Cell Division (drug effects)
• Cell Movement (drug effects)
• Cell Survival (drug effects)
• Endothelium, Vascular (drug effects, pathology)
• Glioma (pathology)
• Humans
• Integrin alphaVbeta3 (antagonists & inhibitors)
• Mice
• Mice, Nude
• Neoplasm Invasiveness (pathology)
• Neoplasm Transplantation
• Neovascularization, Pathologic (pathology)
• Oligopeptides (chemistry, pharmacology)
• Peptides, Cyclic (chemistry, pharmacology)
• Tumor Cells, Cultured (drug effects, pathology)