[Experimental Encephalitozoon cuniculi infection in dexamethasone-immunosuppressed mice].

Abstract

OBJECTIVE:

Microsporidian Encephalitozoon cuniculi has been recognized as an opportunistic pathogen in immunosuppressed individuals, such as AIDS patients. The objective of the study was to develop pharmacologically immunosuppressed animals as a model of the natural occurring E. cuniculi infection.

METHODS:

Distinct groups of adult Balb-C mice were immunosuppressed with different doses of dexamethasone (Dx, 3 or 5 mg/kg/day, intraperitoneal route - IP) and inoculated with E. cuniculi spores by IP route intraperitoneally. Control groups (inoculated animals but non-immunosuppressed and non- inoculated animals but immunosuppressed) were also used. The spores of E. cuniculi were previously cultivated in MDCK cells. The animals were sacrificed and necropsied at 7, 14, 21, 28 and 35 days post-inoculation. Tissue fragments were collected and processed for light microscopy studies, using Gram-chromotrope and hematoxylin-eosin staining techniques.

RESULTS:

In all immunosuppressed and inoculated inoculated immunosuppressed mice,specially in those that received 5 mg/kg/day of dexamethasone, the most prominent necropsy findings were hepatomegaly and splenomegaly. The experimental inoculation resulted in a disseminated non-lethal infection, characterized by granulomatous lesions in several organs (liver, lungs, kidneys, gut and brain) but notably in the hepatic tissue. Spores of E. cuniculi were only seen in few animals treated with 5 mg/kg/day of Dx at 35 days post-infection.

CONCLUSIONS:

Microsporidiosis in Dx-immunosuppressed mice provides a useful model for studies of the microsporidial infection, resembling that one naturally occurring in immunodeficient individuals with AIDS.

• Authors: Maria Anete Lallo, Maurício José dos Santos, Eduardo Fernandes Bondan
• Journal: Revista de saude publica (Rev Saude Publica) Vol. 36 Issue 5 Pg. 621-6 (Oct 2002) ISSN: 0034-8910 [Print] Brazil
• Vernacular Title: Infecção experimental pelo Encephalitozoon cuniculi em camundongos imunossuprimidos com dexametasona.
• PMID: 12471388 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Dexamethasone

Topics

• Animals
• Anti-Inflammatory Agents
• Dexamethasone
• Disease Models, Animal
• Encephalitozoon cuniculi
• Encephalitozoonosis (immunology, pathology)
• Hepatomegaly (parasitology, pathology)
• Immunocompromised Host
• Male
• Mice
• Mice, Inbred BALB C
• Organ Specificity
• Splenomegaly (parasitology, pathology)