Cyclooxygenase-2 expression in fibroblasts and endothelial cells of intestinal polyps.

Abstract	Cyclooxygenase-2 (COX-2), the inducible COX isozyme, plays a key role in intestinal tumorigenesis. We have demonstrated recently that COX-2 protein is induced in the polyp stroma near the intestinal luminal surface in the Apc(Delta716) mouse, a model for human familial adenomatous polyposis, and stimulate tumor angiogenesis. However, the precise cell types that express COX-2 are still to be determined. By immunohistochemical analysis, here we show that the majority of COX-2-expressing cells in the intestinal polyps of Apc(Delta716) mice are fibroblasts and endothelial cells. Furthermore, the COX-2-expressing cells in human familial adenomatous polyposis polyps are also fibroblasts and endothelial cells. In contrast, bone marrow-derived cells such as macrophages and leukocytes express little COX-2 protein in the intestinal polyps. These results clearly indicate that fibroblasts and endothelial cells play important roles in polyp expansion by expressing COX-2, resulting in tumor angiogenesis.
Authors	Masahiro Sonoshita, Kazuaki Takaku, Masanobu Oshima, Ken-ichi Sugihara, Makoto M Taketo
Journal	Cancer research (Cancer Res) Vol. 62 Issue 23 Pg. 6846-9 (Dec 01 2002) ISSN: 0008-5472 [Print] United States
PMID	12460897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Actins Antigens, CD34 Antigens, Differentiation Isoenzymes Membrane Proteins Vimentin monocyte-macrophage differentiation antigen Cyclooxygenase 2 PTGS2 protein, human Prostaglandin-Endoperoxide Synthases
Topics	Actins (biosynthesis) Adenomatous Polyposis Coli (enzymology, metabolism, pathology) Animals Antigens, CD34 (biosynthesis) Antigens, Differentiation (biosynthesis) Cyclooxygenase 2 Endothelium, Vascular (cytology, enzymology, metabolism) Fibroblasts (enzymology, metabolism) Humans Immunohistochemistry Intestinal Polyps (enzymology, pathology) Isoenzymes (biosynthesis) Macrophages (enzymology, metabolism) Membrane Proteins Mice Mice, Knockout Prostaglandin-Endoperoxide Synthases (biosynthesis) Stromal Cells (enzymology, metabolism) Vimentin (biosynthesis)

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