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[Expression of NF-kappa B in human bladder cancer and its clinical significance].

AbstractBACKGROUND & OBJECTIVE:
Studies on solid cancer(such as breast cancer, hepatocellular cancer, pancreatic cancer) indicated that the abnormal expression of nuclear transcription factor Kappa B (NF-kappa B) regulates angiogenesis and cyclin-related genes. This study was designed to investigate the expression differences of NF-kappa B and its regulated genes between human primary transitional cell carcinoma(TCCs) of bladder and non-cancer bladder mucosa and its clinical significance.
METHODS:
Forty-three frozen sections including 30 bladder cancer and 13 non-cancer bladder mucosa were subjected to immunohistochemistry and nucleus staining for determining levels of NF-kappa B family and I kappa B alpha; Five paired cancer and non-cancer specimens were subjected to Western blot for analysis p65, an important subtype of NF-kappa B; Thirteen paired specimens were subjected to RT-PCR for determination mRNA levels of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8.
RESULTS:
Expressions of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8 mRNAs in bladder cancer were higher than that in non-cancer bladder mucosa (P < 0.01, P < 0.05, P < 0.01, P < 0.01, P < 0.05, P < 0.05, P < 0.05 and P < 0.05, respectively). Nucelus stainings of p50, p52, p65, c-Rel, RelB were also stronger in bladder cancer(P < 0.01, P < 0.01, P < 0.01, P < 0.01 and P < 0.01, respectively). Moreover, p65 was expressed more in cancer tissue than that in non-cancer mucosa evidenced by Western blot. In bladder cancer, the ranking score differences of p52, p65, c-Rel protein between lymphatic metastasis group and non-lymphatic metastasis group were statistically significant (P < 0.01, P < 0.01, P < 0.05, respectively).
CONCLUSIONS:
Compared to noncancer bladder mucosa, expressions of NF-kappa B family and its regulated genes in bladder cancer are markedly higher. NF-kappa B may be related to lymphatic metastasis.
AuthorsDong-hua Xie, Xiao-da Tang, Shu-jie Xia, Jian-ming Tan, Xiang-hui Wang, Yong Cai
JournalAi zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 21 Issue 6 Pg. 663-7 (Jun 2002) China
PMID12452071 (Publication Type: Journal Article)
Chemical References
  • I-kappa B Proteins
  • Interleukin-8
  • NF-kappa B
  • NF-kappa B p50 Subunit
  • NFKBIA protein, human
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-rel
  • RELB protein, human
  • Transcription Factor RelA
  • Transcription Factors
  • Cyclin D1
  • NF-KappaB Inhibitor alpha
  • Transcription Factor RelB
Topics
  • Adult
  • Aged
  • Aged, 80 and over
  • Blotting, Western
  • Cyclin D1 (biosynthesis, genetics)
  • Female
  • Humans
  • I-kappa B Proteins (biosynthesis, genetics)
  • Immunohistochemistry
  • Interleukin-8 (biosynthesis, genetics)
  • Male
  • Middle Aged
  • NF-KappaB Inhibitor alpha
  • NF-kappa B (biosynthesis, genetics)
  • NF-kappa B p50 Subunit
  • Proto-Oncogene Proteins (biosynthesis, genetics)
  • Proto-Oncogene Proteins c-rel (biosynthesis, genetics)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factor RelA
  • Transcription Factor RelB
  • Transcription Factors (biosynthesis, genetics)
  • Urinary Bladder Neoplasms (metabolism, pathology)

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