[Expression of NF-kappa B in human bladder cancer and its clinical significance].
Abstract | BACKGROUND & OBJECTIVE: METHODS: Forty-three frozen sections including 30 bladder cancer and 13 non- cancer bladder mucosa were subjected to immunohistochemistry and nucleus staining for determining levels of NF-kappa B family and I kappa B alpha; Five paired cancer and non- cancer specimens were subjected to Western blot for analysis p65, an important subtype of NF-kappa B; Thirteen paired specimens were subjected to RT-PCR for determination mRNA levels of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8. RESULTS: Expressions of p50, p52, p65, c-Rel, RelB, I kappa B alpha, CyclinD1, IL-8 mRNAs in bladder cancer were higher than that in non- cancer bladder mucosa (P < 0.01, P < 0.05, P < 0.01, P < 0.01, P < 0.05, P < 0.05, P < 0.05 and P < 0.05, respectively). Nucelus stainings of p50, p52, p65, c-Rel, RelB were also stronger in bladder cancer(P < 0.01, P < 0.01, P < 0.01, P < 0.01 and P < 0.01, respectively). Moreover, p65 was expressed more in cancer tissue than that in non- cancer mucosa evidenced by Western blot. In bladder cancer, the ranking score differences of p52, p65, c-Rel protein between lymphatic metastasis group and non- lymphatic metastasis group were statistically significant (P < 0.01, P < 0.01, P < 0.05, respectively). CONCLUSIONS:
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Authors | Dong-hua Xie, Xiao-da Tang, Shu-jie Xia, Jian-ming Tan, Xiang-hui Wang, Yong Cai |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 21
Issue 6
Pg. 663-7
(Jun 2002)
China |
PMID | 12452071
(Publication Type: Journal Article)
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Chemical References |
- I-kappa B Proteins
- Interleukin-8
- NF-kappa B
- NF-kappa B p50 Subunit
- NFKBIA protein, human
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-rel
- RELB protein, human
- Transcription Factor RelA
- Transcription Factors
- Cyclin D1
- NF-KappaB Inhibitor alpha
- Transcription Factor RelB
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Blotting, Western
- Cyclin D1
(biosynthesis, genetics)
- Female
- Humans
- I-kappa B Proteins
(biosynthesis, genetics)
- Immunohistochemistry
- Interleukin-8
(biosynthesis, genetics)
- Male
- Middle Aged
- NF-KappaB Inhibitor alpha
- NF-kappa B
(biosynthesis, genetics)
- NF-kappa B p50 Subunit
- Proto-Oncogene Proteins
(biosynthesis, genetics)
- Proto-Oncogene Proteins c-rel
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription Factor RelA
- Transcription Factor RelB
- Transcription Factors
(biosynthesis, genetics)
- Urinary Bladder Neoplasms
(metabolism, pathology)
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