Cell-surface expression and immune receptor recognition of HLA-B27 homodimers.

Abstract

OBJECTIVE:

HLA-B27 is capable of forming in vitro a heavy-chain homodimer structure lacking beta(2)-microglobulin. We undertook this study to ascertain if patients with spondylarthritis express beta(2)-microglobulin-free HLA-B27 heavy chains in the form of homodimers and receptors for HLA-B27 homodimers.

METHODS:

Expression of HLA-B27 heavy chains by mononuclear cells was analyzed by fluorescence-activated cell sorter staining, Western blotting with the monoclonal antibody HC-10, and 2-dimensional isoelectric focusing. Fluorescence-labeled tetrameric complexes of HLA-B27 heavy-chain homodimers were constructed in which each dimer comprised one His-tagged heavy chain and one biotinylated heavy chain, and were used to stain patient and control mononuclear cells and transfected cell lines.

RESULTS:

Patients with spondylarthritis expressed cell-surface HLA-B27 homodimers. Populations of synovial and peripheral blood monocytes, and B and T lymphocytes from patients with spondylarthritis, and controls carried receptors for HLA-B27 homodimers. Experiments with transfected cell lines demonstrated that KIR3DL1 and KIR3DL2, and immunoglobulin-like transcript 4 (ILT4), but not ILT2, are receptors for HLA-B27 homodimers.

CONCLUSION:

Patients with spondylarthritis express both HLA-B27 heavy-chain homodimers and receptors for HLA-B27 homodimers. This may be of significance with regard to disease pathogenesis.

• Authors: Simon Kollnberger, Lucy Bird, Mei-Yi Sun, Christelle Retiere, Veronique M Braud, Andrew McMichael, Paul Bowness
• Journal: Arthritis and rheumatism (Arthritis Rheum) Vol. 46 Issue 11 Pg. 2972-82 (Nov 2002) ISSN: 0004-3591 [Print] United States
• PMID: 12428240 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• HLA-B27 Antigen
• Receptors, Immunologic

Topics

• Adult
• Antigens, Surface (chemistry, immunology)
• Dimerization
• Female
• HLA-B27 Antigen (chemistry, immunology)
• Humans
• Lymphocytes (immunology)
• Male
• Monocytes (immunology)
• Receptors, Immunologic (analysis, immunology)
• Spondylarthritis (immunology)
• Synovial Fluid (cytology)
• T-Lymphocytes (immunology)