Renal antioxidant status in rats with hypertension induced by N sup omega nitro-L-arginine methyl ester.

Abstract

Nitric oxide (NO) has a role in the etiopathogenesis of hypertension. Relaxation of vascular smooth muscles is failed when NO production is reduced leading to increased vascular peripheral resistance. N sup omega nitro-L-arginine methyl ester (L-NAME) is one of the inhibitors of NO production. The aim of this study was to investigate oxidant-antioxidant systems of renal tissue in rats with hypertension induced by L-NAME. Rats were divided into three groups: control group and study groups treated with 100 or 500 mg/l L-NAME in drinking water for 15 days. The activities of catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), and the levels of malondialdehyde (MDA) and NO were studied in the renal tissue after hypertension induction. Arterial blood pressure was increased in both L- NAME groups. CAT activity of 500-mg L-NAME group was higher than control. GSH-Px activity of 500-mg L-NAME group was decreased compared with 100-mg ones. NO level was lower in 500-mg L-NAME group than control. MDA levels in both L-NAME groups were decreased compared with control. In conclusion, hypertension was induced with oral L-NAME treatment. Increased CAT activity was compensated with decreased GSH-Px activity in 500-mg L-NAME group. Both study groups were protected from lipid peroxidation with NO inhibition.

• Authors: Ersin Fadillioglu, Hasan Erdogan, Alaadin Polat, Memet Hanifi Emre
• Journal: Kidney & blood pressure research (Kidney Blood Press Res) Vol. 25 Issue 4 Pg. 211-6 ( 2002) ISSN: 1420-4096 [Print] Switzerland
• PMID: 12424422 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002 S. Karger AG, Basel

Chemical References

• Antioxidants
• Enzyme Inhibitors
• Free Radical Scavengers
• Nitric Oxide
• Malondialdehyde
• Catalase
• Glutathione Peroxidase
• Nitric Oxide Synthase
• Superoxide Dismutase
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Antioxidants (metabolism)
• Blood Pressure (drug effects)
• Catalase (metabolism)
• Enzyme Inhibitors
• Free Radical Scavengers (pharmacology)
• Glutathione Peroxidase (metabolism)
• Hypertension (chemically induced, metabolism)
• Kidney (metabolism)
• Male
• Malondialdehyde (metabolism)
• NG-Nitroarginine Methyl Ester
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase (antagonists & inhibitors)
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)