Effect of Sho-saiko-to extract on hepatic inflammation and fibrosis in dimethylnitrosamine induced liver injury rats.

Abstract

Sho-saiko-to extract, a Chinese herbal medicine, is widely used for treatment of chronic hepatitis in Japan. However, it is not clear what conditions Sho-saiko-to extract improves hepatic inflammation and fibrosis. We therefore induced various stages of liver injury in model rats and administered Sho-saiko-to extract. We then evaluated the liver inflammation and liver fibrosis-improving effects of Sho-saiko-to extract. The liver injury model rats were produced by administration of various doses of dimethylnitrosamine (DMN) and Sho-saiko-to extract was administered to these rats. Then the liver inflammation and fibrosis-improving effects of Sho-saiko-to extract were evaluated according to L-asparate aminotransferase (AST), L-alanine aminotransferase (ALT), liver retinoid levels, levels of hydroxyproline, Transforming Growth Factor-beta (TGF-beta), and the liver fibrosis area. These indicators depended on the total doses of DMN. The ability of Sho-saiko-to extract to improve liver inflammation and fibrosis was limited to the following levels of the respective parameters: AST levels (234-264 U/l), ALT levels (208-232 U/l), TGF-beta levels (1102-1265 pg/g liver tissue), hydroxyproline levels (633-719 nmol/g liver tissue), and liver fibrosis area (9.7-10.6 times for normal rat). These findings suggested that Sho-saiko-to extract is effective in the treatment of liver inflammation and fibrosis up to a certain degree of severity, but it produces no improvement in more severe cases.

• Authors: Masahiko Kusunose, Bing Qiu, Tailin Cui, Atsuhide Hamada, Saburo Yoshioka, Masahide Ono, Mitsuhiko Miyamura, Shojiro Kyotani, Yutaka Nishioka
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 25 Issue 11 Pg. 1417-21 (Nov 2002) ISSN: 0918-6158 [Print] Japan
• PMID: 12419951 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• shosaiko-to
• Dimethylnitrosamine

Topics

• Animals
• Dimethylnitrosamine (toxicity)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (isolation & purification, pharmacology, therapeutic use)
• Inflammation (chemically induced, drug therapy, pathology)
• Liver (drug effects, pathology)
• Liver Cirrhosis, Experimental (chemically induced, drug therapy, pathology)
• Male
• Phytotherapy (methods)
• Rats
• Rats, Wistar