Abstract | BACKGROUND & AIMS: METHODS: RESULTS: Nonsteroidal anti-inflammatory drug ( NSAID) treatment of wild-type mice had minimal effect on the colon. In contrast, NSAID treatment of 4-week-old IL-10(-/-) mice resulted in rapid development of colitis characterized by infiltration of the lamina propria with macrophages and interferon gamma-producing CD4(+) T cells. Colitis persisted after withdrawal of the NSAID. NSAID treatment decreased colonic PGE(2) levels by 75%. Treatment of IL-10(-/-) mice with sulindac sulfone (which does not inhibit PG production) did not induce colitis whereas the NSAID sulindac induced severe colitis. COX-1- or COX-2-selective inhibitors used alone did not induce IBD in IL-10(-/-) mice. However, the combination of COX-1- and COX-2-selective inhibitors did induce colitis. CONCLUSIONS:
NSAID treatment of IL-10(-/-) mice results in the rapid development of severe, chronic IBD. Endogenous PGs are important inhibitors of the development of intestinal inflammation in IL-10(-/-) mice.
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Authors | Daniel J Berg, Juan Zhang, Joel V Weinstock, Hanan F Ismail, Keith A Earle, Hector Alila, Rifat Pamukcu, Steven Moore, Richard G Lynch |
Journal | Gastroenterology
(Gastroenterology)
Vol. 123
Issue 5
Pg. 1527-42
(Nov 2002)
ISSN: 0016-5085 [Print] United States |
PMID | 12404228
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Cyclooxygenase Inhibitors
- Cytokines
- Prostaglandins
- Receptors, Prostaglandin E
- Interleukin-10
- Sulindac
- Interferon-gamma
- Prostaglandin-Endoperoxide Synthases
- sulindac sulfone
- Dinoprostone
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Colitis
(chemically induced, metabolism, pathology)
- Colon
(drug effects, metabolism, pathology)
- Cyclooxygenase Inhibitors
(pharmacology)
- Cytokines
(biosynthesis)
- Dinoprostone
(metabolism)
- Disease Progression
- Interferon-gamma
(biosynthesis)
- Interleukin-10
(deficiency, genetics)
- Longitudinal Studies
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
(genetics)
- Osmolar Concentration
- Phenotype
- Prostaglandin-Endoperoxide Synthases
(metabolism)
- Prostaglandins
(physiology)
- Receptors, Prostaglandin E
(agonists)
- Sulindac
(analogs & derivatives, pharmacology)
- T-Lymphocytes
(metabolism)
- Time Factors
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