Involvement of endothelial cell adhesion molecules in the development of anti-Thy-1 nephritis.

Abstract

To study an involvement of glomerular endothelial cells in the development of anti-Thy-1 nephritis, we examined the expression of endothelial cell adhesion molecules during the course of this model. Ribonuclease protection assay elucidated that expression of mRNA for intercellular adhesion molecule-1 (ICAM-1) was markedly enhanced in the glomeruli with a peak at 2 h (6.5-fold, p < 0.05) after the anti-Thy-1 antibody injection when mesangial cell lysis was recognized and IL-1beta mRNA expression was induced in the glomeruli. The glomerular ICAM-1 was predominantly localized in the endothelial cells and was intensely immunostained at day 1 in the glomerular endothelial cells. In contrast, platelet endothelial cell adhesion molecule-1 (PECAM-1) and vascular endothelial-cadherin mRNA expression increased gradually with a peak at day 6 (2.6-fold (p < 0.05) and 4.2-fold (p < 0.05), respectively) in the glomeruli with mesangial proliferative lesion. PECAM-1 was also immunolocalized in the glomerular endothelial cells and the immunoreactivity was greatly enhanced at day 6. Glomerular expression of vascular cell adhesion molecule-1 and endothelial leukocyte adhesion molecule-1 (E-selectin) was unchanged at a low level during the course of anti-Thy-1 nephritis. Blocking of ICAM-1 by administration of anti-ICAM-1 antibody showed significant decrease in the number of polymorphonuclear leukocytes accumulating in the glomeruli by 45.7% (9.4 +/- 0.2 vs. 5.1 +/- 0.1 per glomerular cross section, p < 0.01) at 2 h. These results suggest a significant involvement of glomerular endothelial cells in the development and repair of anti-Thy-1 nephritis via direct or indirect intercellular interactions between mesangial cells and glomerular endothelial cells.

• Authors: Masato Isome, Hidehiko Fujinaka, Eishin Yaoita, Lili Feng, Laxman P Adhikary, Akira Abe, Satoko Tsuchida, Katsutoshi Kawasaki, Hitoshi Suzuki, Itaru Kihara, Curtis B Wilson, Tadashi Yamamoto
• Journal: Experimental nephrology (Exp Nephrol) Vol. 10 Issue 5-6 Pg. 338-47 ( 2002) ISSN: 1018-7782 [Print] Switzerland
• PMID: 12381918 (Publication Type: Journal Article)
• Copyright: Copyright 2002 S. Karger AG, Basel

Chemical References

• Antibodies
• Cell Adhesion Molecules
• Inflammation Mediators
• Isoantibodies
• RNA, Messenger
• anti-Thy antibody
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Antibodies (pharmacology)
• Cell Adhesion Molecules (genetics, metabolism)
• Endothelium (metabolism)
• Female
• Immunologic Techniques
• Inflammation Mediators (metabolism)
• Intercellular Adhesion Molecule-1 (immunology)
• Isoantibodies (immunology)
• Kidney Glomerulus (metabolism)
• Nephritis (immunology, urine)
• Neutrophil Infiltration (drug effects, immunology)
• Proteinuria (etiology)
• RNA, Messenger (metabolism)
• Rats
• Rats, Inbred WKY