[Preliminary study on the gene expression profiles of oligodendrogliom as with cDNA array].

Abstract

OBJECTIVE:

To study the gene expression profiles of oligodendrogliomas with gene cDNA array.

METHODS:

(32)P tagged cDNA probes converted from the total RNA, which had been extracted from 2 fresh samples of oligodendroglioma and 1 of normal brain tissue, were hybridized with the Atlas array. After washing the membranes, the autoradiography was performed and the autoradiograms were analyzed through the special software.

RESULTS:

As compared to the normal brain tissue, there were 63 co-upregulated genes and 4 co-downregulated genes in these 2 tumor samples. However, a significant quantitative difference existed between them. The expression trend of some genes differed from the known information.

CONCLUSION:

cDNA array is effective for studying the gene expression profiles of oligodendrogliomas and provides new information for the further research on their molecular mechanisms.

• Authors: Rongcai Jiang, Peiyu Pu, Yu Zheng, Chunsheng Kang, Guangxiu Wang, Chunyan Wang
• Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 19 Issue 5 Pg. 379-82 (Oct 2002) ISSN: 1003-9406 [Print] China
• PMID: 12362310 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Brain Neoplasms (genetics, pathology)
• Gene Expression
• Gene Expression Profiling
• Humans
• Oligodendroglioma (genetics, pathology)
• Oligonucleotide Array Sequence Analysis (methods)
• Reverse Transcriptase Polymerase Chain Reaction